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MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea)
- Source :
- Parasite, Vol 26, p 11 (2019), Parasite, Parasite, EDP Sciences, 2019, 26, pp.11. ⟨10.1051/parasite/2019011⟩
- Publication Year :
- 2019
- Publisher :
- EDP Sciences, 2019.
-
Abstract
- Identification of cercariae was long based on morphological and morphometric features, but these approaches remain difficult to implement and require skills that have now become rare. Molecular tools have become the reference even though they remain relatively time-consuming and expensive. We propose a new approach for the identification of cercariae using MALDI-TOF mass spectrometry. Snails of different genera (Radix, Lymnaea, Stagnicola, Planorbis, and Anisus) were collected in the field to perform emitting tests in the laboratory. The cercariae they emitted (Trichobilharzia anseri, Diplostomum pseudospathaceum, Alaria alata, Echinostoma revolutum, Petasiger phalacrocoracis, Tylodelphys sp., Australapatemon sp., Cotylurus sp., Posthodiplostomum sp., Parastrigea sp., Echinoparyphium sp. and Plagiorchis sp.) were characterized by sequencing the D2, ITS2 and ITS1 domains of rDNA, and by amplification using specific Alaria alata primers. A sample of each specimen, either fresh or stored in ethanol, was subjected to a simple preparation protocol for MALDI-TOF analysis. The main spectral profiles were analyzed by Hierarchical Clustering Analysis. Likewise, the haplotypes were analyzed using the maximum likelihood method. Analytical performance and the log-score value (LSV) cut-off for species identification were then assessed by blind testing. The clusters obtained by both techniques were congruent, allowing identification at a species level. MALDI-TOF enables identification at an LSV cut-off of 1.7 without false-positives; however, it requires more data on closely related species. The development of a “high throughput” identification system for all types of cercariae would be of considerable interest in epidemiological surveys of trematode infections.
- Subjects :
- MALDI-TOF
Plagiorchis
food.ingredient
snails
Veterinary (miscellaneous)
030231 tropical medicine
Zoology
Fresh Water
Trematode Infections
Anisus
Digenea
Planorbis
lcsh:Infectious and parasitic diseases
03 medical and health sciences
0302 clinical medicine
food
Species Specificity
Stagnicola
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Animals
lcsh:RC109-216
Echinostoma revolutum
Cercaria
Phylogeny
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health
0303 health sciences
High-throughput identification
biology
030306 microbiology
biology.organism_classification
Furcocercariae
Infectious Diseases
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Haplotypes
Insect Science
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
identification
Animal Science and Zoology
Parasitology
Trematoda
Radix (gastropod)
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 17761042 and 1252607X
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Parasite
- Accession number :
- edsair.doi.dedup.....b30407f5098eacbe140ab82f26caf696