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Crop, genotype, and field environmental conditions shape bacterial and fungal seed epiphytic microbiomes
- Source :
- Canadian Journal of Microbiology. 67:161-173
- Publication Year :
- 2021
- Publisher :
- Canadian Science Publishing, 2021.
-
Abstract
- Seeds are reproductive structures able to carry and transfer microorganisms that play an important role in plant fitness. Genetic and external factors are reported to be partly responsible for the plant microbiome assemblage, but their contribution in seeds is poorly understood. In this study, wheat, canola, and lentil seeds were analyzed to characterize diversity, structure, and persistence of seed-associated microbial communities. Five lines and 2 generations of each crop were subjected to high-throughput amplicon sequencing of the 16S rRNA and internal transcribed spacer (ITS) regions. Bacterial and fungal communities differed most by crop type (30% and 47% of the variance), while generation explained an additional 10% and 15% of the variance. The offspring (i.e., generation harvested in 2016 at the same location) exhibited a higher number of common amplicon sequence variants (ASVs) and less variability in microbial composition. Additionally, in every sample analyzed, a “core microbiome” was detected consisting of 5 bacterial and 12 fungal ASVs. Our results suggest that crop, genotype, and field environmental conditions contributed to the seed-associated microbial assemblage. These findings not only expand our understanding of the factors influencing the seed microbiome but may also help us to manipulate and exploit the microbiota naturally carried by seeds.
- Subjects :
- Crops, Agricultural
Genotype
Microorganism
Immunology
Environment
Biology
Applied Microbiology and Biotechnology
Microbiology
Crop
03 medical and health sciences
RNA, Ribosomal, 16S
DNA, Ribosomal Spacer
Genetics
Microbiome
Molecular Biology
030304 developmental biology
0303 health sciences
Bacteria
030306 microbiology
Microbiota
Fungi
Genetic Variation
High-Throughput Nucleotide Sequencing
food and beverages
General Medicine
Field (geography)
Agronomy
Seeds
Epiphyte
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....b31a96fa3ed96bded1fe8f8d42e062cf
- Full Text :
- https://doi.org/10.1139/cjm-2020-0306