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FtsK translocation on DNA stops at XerCD-dif
- Source :
- Nucleic Acids Research
- Publication Year :
- 2010
-
Abstract
- Escherichia coli FtsK is a powerful, fast, double-stranded DNA translocase, which can strip proteins from DNA. FtsK acts in the late stages of chromosome segregation by facilitating sister chromosome unlinking at the division septum. KOPS-guided DNA translocation directs FtsK towards dif, located within the replication terminus region, ter, where FtsK activates XerCD site-specific recombination. Here we show that FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. Stoppage of translocation at XerCD-dif is accompanied by a reduction in FtsK ATPase and is not associated with FtsK dissociation from DNA. Specific stoppage at recombinase-DNA complexes does not require the FtsKgamma regulatory subdomain, which interacts with XerD, and is not dependent on either recombinase-mediated DNA cleavage activity, or the formation of synaptic complexes.
- Subjects :
- Chromosomal translocation
Genome Integrity, Repair and Replication
Chromosome segregation
03 medical and health sciences
chemistry.chemical_compound
Protein structure
Genetics
Translocase
DNA Cleavage
Binding site
030304 developmental biology
Adenosine Triphosphatases
Recombination, Genetic
0303 health sciences
Binding Sites
Integrases
biology
Escherichia coli Proteins
030302 biochemistry & molecular biology
fungi
Membrane Proteins
Chromosome
DNA
Molecular biology
Protein Structure, Tertiary
Transport protein
Cell biology
Protein Transport
chemistry
biology.protein
Subjects
Details
- Language :
- English
- ISSN :
- 13624962 and 03051048
- Volume :
- 38
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nucleic acids research
- Accession number :
- edsair.doi.dedup.....b3297e23dea83487c01f3785a9a3bb91