Back to Search
Start Over
Oxidative stress promotes hyperandrogenism by reducing sex hormone-binding globulin in polycystic ovary syndrome
- Source :
- Fertility and Sterility. 116:1641-1650
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- To determine the relationships between circulating sex hormone-binding globulin (SHBG) and oxidized low-density lipoprotein (ox-LDL), total oxidant status, total antioxidant capacity, oxidative stress index, malondialdehyde, and the high-density lipoprotein (HDL) inflammatory index in patients with polycystic ovary syndrome (PCOS) and to investigate the effect of oxidative stress on the expression of SHBG and its mechanism in HepG2 cells.Cross-sectional study.University hospital.A total of 533 women with PCOS and 292 control women were included.None.Circulating SHBG, hormones, and metabolic and oxidative stress indices were determined in all subjects. The effects of ox-LDL and ox-HDL on the mRNA and protein expression of SHBG and related transcription factors were observed in HepG2 cells.The HDL inflammatory index, total oxidant status, oxidative stress index, and malondialdehyde levels were significantly higher in the three PCOS subgroups with different SHBG levels than in the controls. The ox-LDL and total antioxidant capacity were higher in the PCOS subgroups with SHBG levels75th percentile compared with the controls or the PCOS subgroup with SHBG levels ≥75th percentile. In HepG2 cells, the SHBG concentration in the culture supernatant, the mRNA levels of SHBG and hepatocyte nuclear factor-4α (HNF-4α), and the protein levels of HNF-4α were significantly lower in ox-LDL- and ox-HDL-treated cells than in the control cells and lipoprotein-treated cells.Oxidative stress inhibits the expression and secretion of SHBG by downregulating HNF-4α in vitro and may be an important factor promoting the occurrence of hyperandrogenemia in PCOS.
- Subjects :
- Adult
medicine.medical_specialty
Adolescent
Globulin
medicine.disease_cause
Young Adult
chemistry.chemical_compound
Sex hormone-binding globulin
Sex Hormone-Binding Globulin
Internal medicine
medicine
Humans
biology
business.industry
Hyperandrogenism
Obstetrics and Gynecology
Hep G2 Cells
medicine.disease
Malondialdehyde
Polycystic ovary
Oxidative Stress
Cross-Sectional Studies
Endocrinology
Reproductive Medicine
chemistry
biology.protein
Female
business
Biomarkers
hormones, hormone substitutes, and hormone antagonists
Oxidative stress
Polycystic Ovary Syndrome
Hormone
Lipoprotein
Subjects
Details
- ISSN :
- 00150282
- Volume :
- 116
- Database :
- OpenAIRE
- Journal :
- Fertility and Sterility
- Accession number :
- edsair.doi.dedup.....b352b62058babe18b194461162ef68e3