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Freezing of air-dried samples of the lichenEvernia prunastri(L.) Ach. ensures that thalli remain healthy for later physiological measurements
- Source :
- Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 147:141-144
- Publication Year :
- 2012
- Publisher :
- Informa UK Limited, 2012.
-
Abstract
- We tested whether freezing of air-dried lichen thalli alter physiological parameters commonly used as valuable stress markers in laboratory and field ecophysiological studies, namely integrity of cell membranes (measured as electrolyte leakage), assimilation pigments (chlorophyll a, chlorophyll b, chlorophyll a+b, total carotenoids content), chlorophyll integrity (OD435/OD415) and chlorophyll a fluorescence emission (expressed as F V/F M, the potential quantum yield of primary photochemistry and PIABS, a global indicator of the photosynthetic performance). Thalli of the lichen Evernia prunastri (L.) Ach. were air-dried, stored in the freezer at ca. −18°C under dark conditions and analysed after short-term storage (15, 30 and 90 days). These periods are compatible with the needs of a relatively rapid data evaluation in biomonitoring studies. After freezing, the investigated parameters showed that lichens remained healthy and suitable for later physiological measurements without biasing data quality.
- Subjects :
- Chlorophyll
Chlorophyll b
chemistry.chemical_classification
Chlorophyll a
Ecology
Evernia prunastri
Evolution
Air pollution
Plant Science
Biology
Photosynthesis
Thallus
Cell membranes
chemistry.chemical_compound
Performance index
Ecology, Evolution, Behavior and Systematics
Behavior and Systematics
chemistry
Botany
Lichen
Carotenoid
Subjects
Details
- ISSN :
- 17245575 and 11263504
- Volume :
- 147
- Database :
- OpenAIRE
- Journal :
- Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
- Accession number :
- edsair.doi.dedup.....b377b21bdde869b1e82b72f97c671b3a