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Chemico-enzymatic synthesis of a new fluorescent-labeled DNA by PCR with a thymidine nucleotide analogue bearing an acridone derivative
- Source :
- Bioorganic & Medicinal Chemistry Letters. 17:776-779
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Triphosphate of a thymidine analogue bearing acridone was prepared and incorporated as a substrate for PCR using KOD Dash DNA polymerase forming a new fluorescent-labeled DNA that is useful as a DNA probe.
- Subjects :
- Photochemistry
DNA polymerase
Clinical Biochemistry
Pharmaceutical Science
DNA-Directed DNA Polymerase
Biochemistry
law.invention
chemistry.chemical_compound
law
Drug Discovery
Nucleotide
Molecular Biology
Polymerase chain reaction
Fluorescent Dyes
chemistry.chemical_classification
biology
Nucleotides
Reverse Transcriptase Polymerase Chain Reaction
RNA-Directed DNA Polymerase
Hybridization probe
Organic Chemistry
DNA
Molecular biology
Acridone
chemistry
biology.protein
Acridines
Molecular Medicine
Indicators and Reagents
Spectrophotometry, Ultraviolet
Thymidine
Subjects
Details
- ISSN :
- 0960894X
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Bioorganic & Medicinal Chemistry Letters
- Accession number :
- edsair.doi.dedup.....b3805dc4f5fa2b21c7375a57c6024c53