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Development of a New Bioprocess for Clean Diosgenin Production through Submerged Fermentation of an Endophytic Fungus
- Source :
- ACS Omega, ACS Omega, Vol 6, Iss 14, Pp 9537-9548 (2021)
- Publication Year :
- 2021
-
Abstract
- Diosgenin is used widely to synthesize steroidal hormone drugs in the pharmaceutical industry. The conventional diosgenin production process, direct acid hydrolysis of the root of Dioscorea zingiberensis C. H. Wright (DZW), causes large amounts of wastewater and severe environmental pollution. To develop a clean and effective method, the endophytic fungus Fusarium sp. CPCC 400226 was screened for the first time for the microbial biotransformation of DZW in submerged fermentation (SmF). Statistical design and response surface methodology (RSM) were implemented to develop the diosgenin production process using the Fusarium strains. The environmental variables that significantly affected diosgenin yield were determined by the two-level Plackett-Burman design (PBD) with nine factors. PBD indicates that the fermentation period, culture temperature, and antifoam reagent addition are the most influential variables. These three variables were further optimized using the response surface design (RSD). A quadratic model was then built by the central composite design (CCD) to study the impact of interaction and quadratic effect on diosgenin yield. The values of the coefficient of determination for the PBD and CCD models were all over 0.95. P-values for both models were 0.0024 and
- Subjects :
- Central composite design
General Chemical Engineering
Environmental pollution
General Chemistry
Diosgenin
Plant use of endophytic fungi in defense
Article
Chemistry
chemistry.chemical_compound
chemistry
Fermentation
Food science
Response surface methodology
Bioprocess
QD1-999
Dioscorea zingiberensis
Subjects
Details
- ISSN :
- 24701343
- Volume :
- 6
- Issue :
- 14
- Database :
- OpenAIRE
- Journal :
- ACS omega
- Accession number :
- edsair.doi.dedup.....b3dbb3a24e2daaeb7c50ee8d67734c83