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Elevated expression of thyroid hormone receptor α 2 (c-erb A- α 2) in nasopharyngeal carcinoma
- Source :
- British Journal of Cancer
- Publication Year :
- 2000
- Publisher :
- Nature Publishing Group, 2000.
-
Abstract
- Differential display was used to identify genes differentially expressed between cultured normal nasal epithelial cells and nasopharyngeal carcinoma (NPC) cell lines. A 130 bp cDNA fragment showing homology with thyroid hormone receptor α2 (TR-α2 or c-erb A-α2) was identified in NPC cell lines. Northern blot analysis using the 130 bp cDNA fragment and a TR-α2 specific cDNA containing part of the coding region as probes, we were able to detect a 2.7 kb transcript corresponding to that of TR-α2 in NPC cell lines but not in normal nasal epithelial cells. RNA in situ hybridization was used to detect TR-α2 expression in clinical biopsies obtained from NPC patients and non-tumour controls. TR-α2 mRNA was detected in 1 out of 24 (4.2%) normal nasopharynx epithelium biopsies, in 5 out of 27 (18.5%) primary and 15 out of 24 (62.5%) recurrent tumours. The positive rate of TR-α2 expression in recurrent NPC biopsies was significantly higher than that in normal nasopharynx epithelium (P
- Subjects :
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
c-erb A-α2 (TR-α2)
Molecular Sequence Data
Biology
Complementary DNA
Sequence Homology, Nucleic Acid
Gene expression
Carcinoma
medicine
otorhinolaryngologic diseases
Tumor Cells, Cultured
Humans
Northern blot
RNA, Neoplasm
In Situ Hybridization
Aged
Differential display
Thyroid hormone receptor
Receptors, Thyroid Hormone
Base Sequence
nasopharyngeal carcinoma
Regular Article
Nasopharyngeal Neoplasms
DNA, Neoplasm
Middle Aged
medicine.disease
Blotting, Northern
Molecular biology
Blot
Gene Expression Regulation, Neoplastic
stomatognathic diseases
Blotting, Southern
Oncology
Nasopharyngeal carcinoma
Female
differential display
RNA in situ hybridization
Subjects
Details
- Language :
- English
- ISSN :
- 15321827 and 00070920
- Volume :
- 83
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- British Journal of Cancer
- Accession number :
- edsair.doi.dedup.....b3f331d6fc06b3c4b089ab36753c696f