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Integration of multiple signaling regulates through apoptosis the differential osteogenic potential of neural crest-derived and mesoderm-derived Osteoblasts
- Source :
- PLoS ONE, Vol 8, Iss 3, p e58610 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Neural crest-derived (FOb) and mesoderm-derived (POb) calvarial osteoblasts are characterized by distinct differences in their osteogenic potential. We have previously demonstrated that enhanced activation of endogenous FGF and Wnt signaling confers greater osteogenic potential to FOb. Apoptosis, a key player in bone formation, is the main focus of this study. In the current work, we have investigated the apoptotic activity of FOb and POb cells during differentiation. We found that lower apoptosis, as measured by caspase-3 activity is a major feature of neural crest-derived osteoblast which also have higher osteogenic capacity. Further investigation indicated TGF-β signaling as main positive regulator of apoptosis in these two populations of calvarial osteoblasts, while BMP and canonical Wnt signaling negatively regulate the process. By either inducing or inhibiting these signaling pathways we could modulate apoptotic events and improve the osteogenic potential of POb. Taken together, our findings demonstrate that integration of multiple signaling pathways contribute to imparting greater osteogenic potential to FOb by decreasing apoptosis.
- Subjects :
- Anatomy and Physiology
Cellular differentiation
Immunofluorescence
lcsh:Medicine
Apoptosis
Signal transduction
Fibroblast growth factor
Mesoderm
Mice
Molecular cell biology
0302 clinical medicine
Osteogenesis
Transforming Growth Factor beta
Signaling in Cellular Processes
lcsh:Science
Musculoskeletal System
Apoptotic Signaling
0303 health sciences
Multidisciplinary
Cell Death
Statistics
Wnt signaling pathway
Signaling cascades
Cell Differentiation
Osteoblast
Cell biology
medicine.anatomical_structure
Neural Crest
030220 oncology & carcinogenesis
Benzamides
Bone Morphogenetic Proteins
Research Article
Histology
Immunology
Dioxoles
Biostatistics
Biology
Bone morphogenetic protein
03 medical and health sciences
Model Organisms
Apoptotic signaling cascade
medicine
Animals
Bone
Immunoassays
030304 developmental biology
Osteoblasts
Wnt signaling cascade
lcsh:R
Transforming growth factor beta
Wnt Proteins
TGF-beta signaling cascade
Immunologic Techniques
biology.protein
lcsh:Q
Mathematics
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....b40613e4910455a9e724a6bb145b8a5d