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Cytofluorometric DNA ploidy analysis in giant cell tumor of bone: histologic and prognostic value
- Source :
- Cancer Letters. 136:223-229
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- DNA ploidy analysis by DNA cytofluorometry was performed on 41 tumors obtained from 37 patients with primary giant cell tumor of bone (GCT). Histologically, 26 of the tumors from primary or recurrent lesions were evaluated as grade I, and 13 tumors as grade II. Among the 33 primary GCT patients, 4 patients had local recurrence or pulmonary metastasis. The DNA ploidy pattern and the percentage of hyperdiploid cells showing a greater DNA content than diploid cells, were obtained from DNA cytofluorometry. All of the 33 primary tumors were diploid. Of 6 recurrent tumors, 4 were diploid and 2 were euploid-polyploid. One of the two pulmonary metastatic tumors was diploid, but another that demonstrated a malignant transformation to malignant fibrous histiocytoma was aneuploid. The percentage of hyperdiploid cells was significantly different between primary and recurrent tumors (P = 0.0188) and between grade I and grade II tumors (P = 0.0052), while there was no difference between primary tumors in the cases that recurred or metastasized and those that did not. Thus, these data indicate that cell proliferative activity is closely correlated with biological aggressiveness and histological grading, although DNA ploidy is not useful for predicting prognosis.
- Subjects :
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Adolescent
Cell Proliferative Activity
Bone Neoplasms
Biology
Malignant transformation
chemistry.chemical_compound
Predictive Value of Tests
medicine
Humans
Fluorometry
Grading (tumors)
Dna ploidy
Aged
Giant Cell Tumor of Bone
DNA
Middle Aged
Aneuploidy
Prognosis
medicine.disease
Diploidy
Microscopy, Fluorescence
Oncology
chemistry
Female
Ploidy
Cell Division
Giant-cell tumor of bone
DNA ploidy analysis
Subjects
Details
- ISSN :
- 03043835
- Volume :
- 136
- Database :
- OpenAIRE
- Journal :
- Cancer Letters
- Accession number :
- edsair.doi.dedup.....b433632dbc694c1b90b24a3228906c52