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Extraction, preparation and an assessment of the activity of carboxymethyl polysaccharide from Panax japonicus
- Source :
- Food Science and Technology, Issue: ahead, Published: 18 OCT 2021, Food Science and Technology (2021), Food Science and Technology, Volume: 42, Article number: e82221, Published: 18 OCT 2021, Food Science and Technology v.42 2022, Food Science and Technology (Campinas), Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA), instacron:SBCTA
- Publication Year :
- 2021
- Publisher :
- Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2021.
-
Abstract
- A response surface method (RSM) was employed to optimize polysaccharide extraction from Panax japonicus (PJPS). Also, carboxymethyl substitution of PJPS (CM-PJPS) was studied using a response surface methodology. A three-variable Box–Behnken design (BBD) methodology was applied. RSM analyses revealed that conditions that maximized polysaccharide yield were as follows: a liquid-to-material ratio 22:1, an extraction temperature of 83.99 °C and an extraction duration of 2.32 h. Under optimum extraction conditions, the average absorbance value of PJPS was 0.6001, and the purity of PJPS was 98.2%. A quadratic regression model for CM-PJPS was obtained via BBD. The optimum extraction conditions for the process were determined to include a 1.38 h reaction time, 1.24 g monochloroacetic acid (MCA) and a reaction temperature of 52.85 °C. Under optimum extraction conditions, the average degree of CM-PJPS substitution was 0.9733. The structure of the PJPS was examined by means of Fourier transform infrared spectroscopy (FTIR). CM-PJPS exhibited the strongest anti-oxidant activity in vitro. In addition, CM-PJPS inhibited Skov3 and A2780 cancer cell proliferation. Our data revealed that CM-PJPS is a promising natural antioxidant with potential value as a food supplement and for treatment of cancer.
- Subjects :
- chemistry.chemical_classification
Antioxidant
Chromatography
Nutrition. Foods and food supply
Chemistry
medicine.medical_treatment
Extraction (chemistry)
antioxidant activity
Panax japonicus
Polysaccharide
Absorbance
Yield (chemistry)
polysaccharide
medicine
T1-995
TX341-641
Response surface methodology
Fourier transform infrared spectroscopy
Technology (General)
carboxymethylation
Food Science
Biotechnology
response surface
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Food Science and Technology, Issue: ahead, Published: 18 OCT 2021, Food Science and Technology (2021), Food Science and Technology, Volume: 42, Article number: e82221, Published: 18 OCT 2021, Food Science and Technology v.42 2022, Food Science and Technology (Campinas), Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA), instacron:SBCTA
- Accession number :
- edsair.doi.dedup.....b480a45109c983a5234ff6385a44c779