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Genome resources for climate-resilient cowpea, an essential crop for food security
- Source :
- The Plant journal : for cell and molecular biology, vol 89, iss 5
- Publication Year :
- 2017
- Publisher :
- eScholarship, University of California, 2017.
-
Abstract
- Summary Cowpea (Vigna unguiculata L. Walp.) is a legume crop that is resilient to hot and drought-prone climates, and a primary source of protein in sub-Saharan Africa and other parts of the developing world. However, genome resources for cowpea have lagged behind most other major crops. Here we describe foundational genome resources and their application to analysis of germplasm currently in use in West African breeding programs. Resources developed from the African cultivar IT97K-499-35 include a whole-genome shotgun (WGS) assembly, a bacterial artificial chromosome (BAC) physical map, and assembled sequences from 4,355 BACs. These resources and WGS sequences of an additional 36 diverse cowpea accessions supported the development of a genotyping assay for 51,128 SNPs, which was then applied to five biparental RIL populations to produce a consensus genetic map containing 37,372 SNPs. This genetic map enabled the anchoring of 100 Mb of WGS and 420 Mb of BAC sequences, an exploration of genetic diversity along each linkage group, and clarification of macrosynteny between cowpea and common bean. The SNP assay enabled a diversity analysis of materials from West African breeding programs. Two major subpopulations exist within those materials, one of which has significant parentage from South and East Africa and more diversity. There are genomic regions of high differentiation between subpopulations, one of which coincides with a cluster of nodulin genes. The new resources and knowledge help to define goals and accelerate the breeding of improved varieties to address food security issues related to limited-input small-holder farming and climate stress. This article is protected by copyright. All rights reserved.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Germplasm
Chromosomes, Artificial, Bacterial
Climate
Plant Biology
BAC sequencing
Plant Science
01 natural sciences
Genome
Food Supply
iSelect genotyping array
Food security
genetic anchoring
consensus genetic map
synteny
Bacterial
food and beverages
WGS sequencing
Artificial
Zero Hunger
Genome, Plant
Biotechnology
Crops, Agricultural
Genotype
Plant Biology & Botany
Genomics
Crops
Biology
Chromosomes, Plant
Chromosomes
03 medical and health sciences
Vigna unguiculata L. Walp
West Africa
Genetics
Plant breeding
Synteny
Genetic diversity
Agricultural
business.industry
Vigna
Phaseolus vulgaris L
Cell Biology
Plant
cowpea
030104 developmental biology
Agriculture
Biochemistry and Cell Biology
business
010606 plant biology & botany
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biology, vol 89, iss 5
- Accession number :
- edsair.doi.dedup.....b4910102ba733e51e349c6a5cea8a6a4