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Localization of oxidized phosphatidylcholine in nonalcoholic fatty liver disease: Impact on disease progression
- Source :
- Hepatology. 43:506-514
- Publication Year :
- 2006
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2006.
-
Abstract
- Nonalcoholic steatohepatitis/nonalcoholic fatty liver disease is considered to be a hepatic manifestation of various metabolic disorders. However, its precise pathogenic mechanism is obscure. Oxidative stress and consequent lipid peroxidation seem to play a pivotal role in disease progression. In this study, we analyzed the localization of oxidized phosphatidylcholine (oxPC), a lipid peroxide that serves as a ligand for scavenger receptors, in livers of patients with this steatotic disorder. Specimens of non-alcoholic fatty liver disease (15 autopsy livers with simple steatosis and 32 biopsy livers with steatohepatitis) were examined via immunohistochemistry and immunoelectron microscopy using a specific antibody against oxPC. In addition, scavenger receptor expression, hepatocyte apoptosis, iron deposition, and inflammatory cell infiltration in the diseased livers were also assessed. Oxidized phosphatidylcholine was mainly localized to steatotic hepatocytes and some macrophages/Kupffer cells. A few degenerative or apoptotic hepatocytes were also positive for oxPC. Immunoelectron microscopy showed oxPC localized to cytoplasmic/intracytoplasmic membranes including lipid droplets. Steatotic livers showed enhanced expression of scavenger receptors. The number of oxPC cells was correlated with disease severity and the number of myeloperoxidase-positive neutrophils, but not with the degree of iron deposition. In conclusion, distinct localization of oxPC in liver tissues suggest that neutrophil myeloperoxidase-derived oxidative stress may be crucial in the formation of oxPC and the progression of steatotic liver disease.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Iron
Immunoelectron microscopy
Apoptosis
Biology
medicine.disease_cause
Lipid droplet
Nonalcoholic fatty liver disease
medicine
Humans
Scavenger receptor
Aged
Hepatology
Lipid peroxide
Fatty liver
Middle Aged
medicine.disease
Fatty Liver
Oxidative Stress
Neutrophil Infiltration
Disease Progression
Hepatocytes
Phosphatidylcholines
Female
Lipid Peroxidation
Steatohepatitis
Oxidative stress
Subjects
Details
- ISSN :
- 15273350 and 02709139
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Hepatology
- Accession number :
- edsair.doi.dedup.....b4954bd623deeb0cd162bb24d6987ce4