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R2R3-MYB genes coordinate conical cell development and cuticular wax biosynthesis in Phalaenopsis aphrodite
- Source :
- Plant Physiology
- Publication Year :
- 2021
- Publisher :
- Oxford University Press, 2021.
-
Abstract
- Petals of the monocot Phalaenopsis aphrodite (Orchidaceae) possess conical epidermal cells on their adaxial surfaces, and a large amount of cuticular wax is deposited on them to serve as a primary barrier against biotic and abiotic stresses. It has been widely reported that subgroup 9A members of the R2R3-MYB gene family, MIXTA and MIXTA-like in eudicots, act to regulate the differentiation of conical epidermal cells. However, the molecular pathways underlying conical epidermal cell development and cuticular wax biosynthesis in monocot petals remain unclear. Here, we characterized two subgroup 9A R2R3-MYB genes, PaMYB9A1 and PaMYB9A2 (PaMYB9A1/2), from P. aphrodite through the transient overexpression of their coding sequences and corresponding chimeric repressors in developing petals. We showed that PaMYB9A1/2 function to coordinate conical epidermal cell development and cuticular wax biosynthesis. In addition, we identified putative targets of PaMYB9A1/2 through comparative transcriptome analyses, revealing that PaMYB9A1/2 acts to regulate the expression of cell wall-associated and wax biosynthetic genes. Furthermore, a chemical composition analysis of cuticular wax showed that even-chain n-alkanes and odd-chain primary alcohols are the main chemical constituents of cuticular wax deposited on petals, which is inconsistent with the well-known biosynthetic pathways of cuticular wax, implying a distinct biosynthetic pathway occurring in P. aphrodite flowers. These results reveal that the function of subgroup 9A R2R3-MYB family genes in regulating the differentiation of epidermal cells is largely conserved in monocots and dicots. Furthermore, both PaMYB9A1/2 have evolved additional functions controlling the biosynthesis of cuticular wax.<br />Two subgroup 9A R2R3-MYB genes act to regulate the development of conical epidermal cells and the biosynthesis of cuticular wax in Phalaenopsis petals.
- Subjects :
- AcademicSubjects/SCI01280
Physiology
Plant Science
Flowers
Biology
Genes, Plant
Plant Epidermis
Transcriptome
Phalaenopsis aphrodite
Gene Expression Regulation, Plant
Genetics
Morphogenesis
Gene family
MYB
Orchidaceae
Gene
Research Articles
Cell Proliferation
Wax
AcademicSubjects/SCI01270
integumentary system
AcademicSubjects/SCI02288
AcademicSubjects/SCI02287
fungi
AcademicSubjects/SCI02286
food and beverages
Cell Differentiation
Genes, Development and Evolution
biology.organism_classification
Plants, Genetically Modified
Cell biology
visual_art
Waxes
visual_art.visual_art_medium
Petal
Function (biology)
Subjects
Details
- Language :
- English
- ISSN :
- 15322548 and 00320889
- Volume :
- 188
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Plant Physiology
- Accession number :
- edsair.doi.dedup.....b4b0bcf7c33bc06c5508517a4caedf82