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A microsatellite map of the African human malaria vector Anopheles funestus
- Source :
- Journal of Heredity, Journal of Heredity, Oxford University Press (OUP), 2004, 95 (1), pp.29-34. ⟨10.1093/jhered/esh011⟩
- Publication Year :
- 2004
- Publisher :
- HAL CCSD, 2004.
-
Abstract
- Microsatellite markers and chromosomal inversion polymorphisms are useful genetic markers for determining population structure in Anopheline mosquitoes. In Anopheles funestus (2N = 6), only chromosome arms 2R, 3R, and 3L are known to carry polymorphic inversions. The physical location of microsatellite markers with respect to polymorphic inversions is potentially important information for interpreting population genetic structure, yet none of the available marker sets have been physically mapped in this species. Accordingly, we mapped 32 polymorphic A. funestus microsatellite markers to the polytene chromosomes using fluorescent in situ hybridization (FISH) and identified 16 markers outside of known polymorphic inversions. Here we provide an integrated polytene chromosome map for A. funestus that includes the breakpoints of all known polymorphic inversions as well as the physical locations of microsatellite loci developed to date. Based on this map, we suggest a standard set of 16 polymorphic microsatellite markers that are distributed evenly across the chromosome complement, occur predominantly outside of inversions, and amplify reliably. Adoption of this set by researchers working in different regions of Africa will facilitate metapopulation analyses of this primary malaria vector.
- Subjects :
- 030231 tropical medicine
Population
Biology
Disease Vectors
03 medical and health sciences
0302 clinical medicine
parasitic diseases
Anopheles
Genetics
Polymorphic Microsatellite Marker
Animals
Humans
education
Molecular Biology
Genetics (clinical)
In Situ Hybridization, Fluorescence
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Chromosomal inversion
0303 health sciences
education.field_of_study
Polytene chromosome
Chromosome
Chromosome Mapping
3. Good health
Malaria
Genetic marker
Genetic structure
Africa
[SDE]Environmental Sciences
Microsatellite
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Biotechnology
Microsatellite Repeats
Subjects
Details
- Language :
- English
- ISSN :
- 00221503
- Database :
- OpenAIRE
- Journal :
- Journal of Heredity, Journal of Heredity, Oxford University Press (OUP), 2004, 95 (1), pp.29-34. ⟨10.1093/jhered/esh011⟩
- Accession number :
- edsair.doi.dedup.....b4cad906fc8e9b227b2a42fc73b6366b