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Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury
- Source :
- Neural Regeneration Research, Neural Regeneration Research, Vol 16, Iss 2, Pp 350-356 (2021)
- Publication Year :
- 2020
- Publisher :
- Wolters Kluwer - Medknow, 2020.
-
Abstract
- Flavonoids have been reported to have therapeutic potential for spinal cord injury. Hawthorn leaves have abundant content and species of total flavonoids, and studies of the effects of the total flavonoids of hawthorn leaves on spinal cord injury have not been published in or outside China. Therefore, Sprague-Dawley rats were used to establish a spinal cord injury model by Allen's method. Rats were intraperitoneally injected with 0.2 mL of different concentrations of total flavonoids of hawthorn leaves (5, 10, and 20 mg/kg) after spinal cord injury. Injections were administered once every 6 hours, three times a day, for 14 days. After treatment with various concentrations of total flavonoids of hawthorn leaves, the Basso, Beattie, and Bresnahan scores and histological staining indicated decreases in the lesion cavity and number of apoptotic cells of the injured spinal cord tissue; the morphological arrangement of the myelin sheath and nerve cells tended to be regular; and the Nissl bodies in neurons increased. The Basso, Beattie, and Bresnahan scores of treated spinal cord injury rats were increased. Western blot assays showed that the expression levels of pro-apoptotic Bax and cleaved caspase-3 were decreased, but the expression level of the anti-apoptotic Bcl-2 protein was increased. The improvement of the above physiological indicators showed a dose-dependent relationship with the concentration of total flavonoids of hawthorn leaves. The above findings confirm that total flavonoids of hawthorn leaves can reduce apoptosis and exert neuroprotective effects to promote the recovery of the motor function of rats with spinal cord injury. This study was approved by the Ethics Committee of the Guangxi Medical University of China (approval No. 201810042) in October 2018.
- Subjects :
- 0301 basic medicine
cleaved caspase-3
total flavonoids of hawthorn leaves
Inflammation
Pharmacology
Neuroprotection
lcsh:RC346-429
Lesion
03 medical and health sciences
symbols.namesake
0302 clinical medicine
Developmental Neuroscience
Western blot
motor function recovery
medicine
Spinal cord injury
lcsh:Neurology. Diseases of the nervous system
medicine.diagnostic_test
business.industry
apoptosis
Spinal cord
medicine.disease
spinal cord injury
Nissl bodies
030104 developmental biology
medicine.anatomical_structure
Apoptosis
inflammation
Nissl body
symbols
bax protein
bcl-2 protein
neuroprotection
nissl bodies
medicine.symptom
business
Bax protein
030217 neurology & neurosurgery
Research Article
Bcl-2 protein
Subjects
Details
- Language :
- English
- ISSN :
- 18767958 and 16735374
- Volume :
- 16
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Neural Regeneration Research
- Accession number :
- edsair.doi.dedup.....b4fef17c7a15899a562c02fb4ba6ed9c