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Quercetin ameliorates testosterone secretion disorder by inhibiting endoplasmic reticulum stress through the miR-1306-5p/HSD17B7 axis in diabetic rats
- Source :
- Bosnian Journal of Basic Medical Sciences (2021)
- Publication Year :
- 2021
-
Abstract
- Testicular damage and testosterone secretion disorder are associated with diabetes mellitus. Quercetin, a common flavonoid, has antioxidant, anti-cancer, and blood sugar lowering effects. Therefore, this study aims to investigate the effect of quercetin on the reproductive system of male rats with diabetes in vivo and in vitro and elucidate its mechanism. Streptozotocin (STZ) induction was used to establish a diabetes model in forty male Sprague Dawley (SD) rats, which were subsequently administered with 20 or 50 mg/kg of quercetin. Leydig cells of rat testes were treated by high glucose (HG) followed by 5 or 10 μM quercetin. Two doses of quercetin increased rat body weight and testicular weight, decreased blood glucose,and inhibited oxidative stress. RT-qPCR and Western blotting revealed that quercetin alleviated STZ-induced testicular damage and promoted testosterone synthesis. Both doses of quercetin reduced ROS and MDA levels, and increased SOD level in HG-treated cells. Both, in vivo and in vitro results confirmed that a high dose of quercetin was more effective. MiR-1306-5p was upregulated in testicular tissue of diabetic rats and HG-treated cells. 17β-hydroxysteroid dehydrogenase (HSD17B7) was a target of miR-1306-5p and HSD17B7 was downregulated in STZ-induced rat tissues and HG-treated cells. HSD17B7 overexpression reversed the increase of C/EBP homologous protein (CHOP) and glucose-regulated protein 78 (Grp78) protein levels as well as eIF2α phosphorylation level and promotion of cell apoptosis caused by miR-1306-5p overexpression. Moreover, overexpression of HSD17B7 activated the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) axis in HG-treated cells. In conclusion, quercetin inhibits ER stress and improves testosterone secretion disorder through the miR-1306-5p/HSD17B7 axis in diabetic rats.
- Subjects :
- the JAK2/STAT3 axis
Male
HSD17B7
Medicine (General)
medicine.medical_specialty
Blood sugar
Apoptosis
medicine.disease_cause
Diabetes Mellitus, Experimental
Rats, Sprague-Dawley
chemistry.chemical_compound
R5-920
Downregulation and upregulation
Internal medicine
Diabetes mellitus
medicine
Animals
heterocyclic compounds
Testosterone
STAT3
biology
business.industry
General Medicine
miR-1306-5p
Streptozotocin
medicine.disease
Endoplasmic Reticulum Stress
Rats
MicroRNAs
Oxidative Stress
Endocrinology
chemistry
biology.protein
Quercetin
business
Oxidative stress
medicine.drug
Subjects
Details
- ISSN :
- 18404812
- Volume :
- 22
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Bosnian journal of basic medical sciences
- Accession number :
- edsair.doi.dedup.....b502bdff0cd660870996b3100f7a19d1