Back to Search
Start Over
Study of manganese binding to the ferroxidase centre of human H-type ferritin
- Source :
- Journal of inorganic biochemistry 182 (2018): 103–112. doi:10.1016/j.jinorgbio.2018.02.003, info:cnr-pdr/source/autori:Ardini M.; Howes B.D.; Fiorillo A.; Falvo E.; Sottini S.; Rovai D.; Lantieri M.; Ilari A.; Gatteschi D.; Spina G.; Chiancone E.; Stefanini S.; Fittipaldi M./titolo:Study of manganese binding to the ferroxidase centre of human H-type ferritin/doi:10.1016%2Fj.jinorgbio.2018.02.003/rivista:Journal of inorganic biochemistry/anno:2018/pagina_da:103/pagina_a:112/intervallo_pagine:103–112/volume:182
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Ferritins are ubiquitous and conserved proteins endowed with enzymatic ferroxidase activity, that oxidize Fe(II) ions at the dimetal ferroxidase centre to form a mineralized Fe(III) oxide core deposited within the apo-protein shell. Herein, the in vitro formation of a heterodimetal cofactor constituted by Fe and Mn ions has been investigated in human H ferritin (hHFt). Namely, Mn and Fe binding at the hHFt ferroxidase centre and its effects on Fe(II) oxidation have been investigated by UV–Vis ferroxidation kinetics, fluorimetric titrations, multifrequency EPR, and preliminary Mossbauer spectroscopy. Our results show that in hHFt, both Fe(II) and Mn(II) bind the ferroxidase centre forming a Fe-Mn cofactor. Moreover, molecular oxygen seems to favour Mn(II) binding and increases the ferroxidation activity of the Mn-loaded protein. The data suggest that Mn influences the Fe binding and the efficiency of the ferroxidation reaction. The higher efficiency of the Mn-Fe heterometallic centre may have a physiological relevance in specific cell types (i.e. glia cells), where the concentration of Mn is the same order of magnitude as iron.
- Subjects :
- 0301 basic medicine
Human H ferritin
Kinetics
Ferroxidase activity
Biochemistry
Cofactor
law.invention
Inorganic Chemistry
03 medical and health sciences
law
Mössbauer spectroscopy
Heterodimetal manganese-iron centre
Humans
Electron paramagnetic resonance
Manganese
biology
Chemistry
Electron Spin Resonance Spectroscopy
Ceruloplasmin
Ferroxidase centre
EPR
Mössbauer
Apoferritins
Protein Binding
Ferritin
Crystallography
030104 developmental biology
biology.protein
Titration
Subjects
Details
- ISSN :
- 01620134
- Volume :
- 182
- Database :
- OpenAIRE
- Journal :
- Journal of Inorganic Biochemistry
- Accession number :
- edsair.doi.dedup.....b506ebd3f9aeb70377af59a28088bb1d