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Expression and hormone regulation of prolactin receptors in rat dorsal and lateral prostate
- Source :
- Endocrinology. 137:3078-3088
- Publication Year :
- 1996
- Publisher :
- The Endocrine Society, 1996.
-
Abstract
- We have studied the receptors that presumably mediate the biological effects of PRL in rat dorsal (DP) and lateral (LP) prostate. The PRL receptor proteins were localized to the glandular secretory epithelium of prostatic tissue by immunohistochemistry. Both the short and the long PRL receptor proteins were detected in DP and LP by Western blot analysis and cross-linking of [125I]human PRL to membrane preparations of DP and LP. Three messenger RNAs (mRNAs) for the long [1.3-1.7, 2.5, and 9.5-10 kilobases (kb)] and short (0.6-0.7, 3.0-4.6, and 10-12 kb) PRL receptors were expressed in dorsal and lateral lobes of rat prostate. Testosterone (T), estrogen (E), and PRL regulation of PRL receptor expression in rat DP and LP was studied in organ culture, which has been shown to be a suitable model to study hormone responses of prostatic tissue in vitro. The mRNAs of the short and long PRL receptors were differentially regulated in rat dorsolateral prostate. T, E, and PRL regulated the level of the long PRL receptor mRNAs in a tissue-specific manner, whereas hormone regulation of the short PRL receptor mRNAs was only modest. Furthermore, the hormonal responses of the different mRNA splicing variants of the long PRL receptor were not all similar; T, E, and PRL each increased the expression of 1.3- to 1.7-kb and 9.5- to 10-kb transcripts in DP, but only T did so in LP, whereas no clear regulation for the 2.5-kb mRNA could be observed in either tissue. This suggests that the hormonal regulation occurs at least at the posttranscriptional level. The effects of T and E were counteracted by the antihormones cyproterone and toremifene, respectively, indicating a specific receptor-mediated manner of steroid action.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
Transcription, Genetic
Receptors, Prolactin
medicine.drug_class
Receptor expression
Biology
Rats, Sprague-Dawley
Organ Culture Techniques
Endocrinology
Internal medicine
medicine
Animals
Humans
Testosterone
RNA, Messenger
Cyproterone
Receptor
Messenger RNA
Estradiol
Cell Membrane
Prostate
Blotting, Northern
Immunohistochemistry
Epithelium
Prolactin
Rats
Blot
medicine.anatomical_structure
Estrogen
Toremifene
DNA Probes
hormones, hormone substitutes, and hormone antagonists
Hormone
Subjects
Details
- ISSN :
- 19457170 and 00137227
- Volume :
- 137
- Database :
- OpenAIRE
- Journal :
- Endocrinology
- Accession number :
- edsair.doi.dedup.....b507b8c3cc473de22fc6e756686a6ba1
- Full Text :
- https://doi.org/10.1210/endo.137.7.8770934