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Radio-protective potential of lipoic acid free and nano-capsule against 99mTc-MIBI induced injury in cardio vascular tissue
- Source :
- Journal of X-ray science and technology. 27(1)
- Publication Year :
- 2018
-
Abstract
- Background SPECT MPI (Single photon emission computed tomography myocardial perfusion imaging) is an essential tool for diagnosis of cardiovascular disease, but it also involves considerable exposure to ionizing radiation. Objective To determine the radioprotective potential of lipoic acid free and nano-capsule against 99mTc-MIBI-induced injury in cardiovascular tissue. Methods The radioprotective ability was assessed by blood count, histopathology and heart enzymes in different groups of mice. Hearts of mice from all groups were dissected and prepared for oxidative stress analysis of superoxide dismutase (SOD) and malondialdehyde (MDA). Furthermore, levels of DNA damage in heart and bone marrow cells were evaluated by alkaline comet assay technique. The same measurements were estimated after treating the mice with lipoic acid. Results Comparing mice injected by radiopharmaceutics with control group showed a significant depression in the count of white blood cells (WBC) by about 40 % at 24 &72 hrs post-radiopharmaceutical administration. Moreover, platelets count was decreased by 27% at 72 hrs post-radiopharmaceutical administration. Radiation also dropped in super oxide dismutase (SOD) and increased in activity of heart enzymes and level of MDA (Malondialdehyde). Additionally, histopathological observation was characterized by focal necrosis of cardiac myocytes. 99mTc-MIBI induced DNA damage had significant increase. Nevertheless, pretreatment with free and lipoic acid nano-capsules (LANC's) prevented the reduction induced in WBCs and platelets, and improved their counts significantly. Conversely pre-treatment with lipoic acid free and nano-capsule significantly increased the activity of SOD and decreased the level of MDA and therefore protected the cardiovascular tissues and reduced DNA strand-break, consequently and enhanced the body weight of the mice. Conclusions These findings highlight the efficacy of lipoic acid free and nano-capsule as a radio protector.
- Subjects :
- Male
medicine.medical_specialty
Antioxidant
DNA damage
medicine.medical_treatment
Radiation-Protective Agents
Pharmacology
Antioxidants
030218 nuclear medicine & medical imaging
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Leukocyte Count
Mice
0302 clinical medicine
Nanocapsules
Medicine
Animals
Radiology, Nuclear Medicine and imaging
Electrical and Electronic Engineering
Instrumentation
Mice, Inbred BALB C
Radiation
Super oxide dismutase
biology
Thioctic Acid
business.industry
Platelet Count
Myocardium
Heart
Organotechnetium Compounds
Condensed Matter Physics
Malondialdehyde
Lipoic acid
medicine.anatomical_structure
chemistry
030220 oncology & carcinogenesis
biology.protein
Histopathology
Bone marrow
Radiopharmaceuticals
business
DNA Damage
Subjects
Details
- ISSN :
- 10959114
- Volume :
- 27
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of X-ray science and technology
- Accession number :
- edsair.doi.dedup.....b58386bbdf1e00082700bbb3020d537a