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Receptor usage by the Acanthocheilonema viteae-derived immunomodulator, ES-62
- Source :
- Experimental Parasitology. 132:97-102
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- ES-62 is an immunomodulatory phosphorylcholine (PC)-containing glycoprotein secreted by the rodent filarial nematode Acanthocheilonema viteae. Previously, the use of knockout mice has revealed the effects of ES-62 on macrophages and dendritic cells to be dependent on TLR4. However, it is possible that ES-62 may interact with additional proteins on the surfaces of target cells and hence that cells may vary with respect to receptor usage. In this study, we identified by molecular weight, proteins that interact with ES-62 and found differences amongst the immune system cells studied. Thus, whereas lymphocytes appear to have two major interacting proteins of ∼135 and ∼82 kDa, U937 monocytes only contain an ES-62-binding protein of the latter molecular weight. Binding to the proteins on B cells and U937 cells was blocked by PC, suggesting a critical role for this ES-62 moiety in facilitating interaction. Finally, ES-62 binding is followed by internalization in both macrophages and B cells but only in the former was absence of TLR4 found to block internalization. These findings are consistent with differences in receptor usage by ES-62 amongst different cell-types.
- Subjects :
- RM
T-Lymphocytes
media_common.quotation_subject
Blotting, Western
Immunology
Receptors, Cell Surface
Biosensing Techniques
Dipetalonema
Monocytes
Jurkat Cells
Mice
Immune system
Animals
Humans
Receptor
Internalization
media_common
chemistry.chemical_classification
B-Lymphocytes
Mice, Inbred BALB C
Acanthocheilonema viteae
biology
U937 cell
Phosphorylcholine
Macrophages
Cell Membrane
Helminth Proteins
U937 Cells
General Medicine
biology.organism_classification
Mice, Inbred C57BL
Molecular Weight
Toll-Like Receptor 4
Infectious Diseases
Biochemistry
chemistry
TLR4
Parasitology
Glycoprotein
Subjects
Details
- ISSN :
- 00144894
- Volume :
- 132
- Database :
- OpenAIRE
- Journal :
- Experimental Parasitology
- Accession number :
- edsair.doi.dedup.....b5935a862dcd61eb9a7bf23f8e280efe