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Next generation sequencing in cytology
- Source :
- Cytopathology
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high‐quality DNA for NGS analysis, especially for predicting patients' response to targeted treatments and for refining the risk of malignancy in indeterminate cytological diagnoses. However, several pre‐analytical factors may influence the reliability of NGS clinical analysis. Here, we briefly review the challenges of NGS in cytology practice, focusing on those pre‐analytical factors that may negatively affect NGS success rates and routine diagnostic applications. Finally, we address the future directions of the field.<br />Molecular cytopathology is a rapidly evolving field. Modern molecular cytopathologists play a key role in bridging the gap between conventional microscopy and novel molecular technologies. Cytological samples represent a valid source of high‐quality DNA for next generation sequencing (NGS) analysis, especially for predicting patients’ response to targeted treatments and for refining the risk of malignancy in indeterminate cytological diagnosis.
- Subjects :
- Histology
Cytodiagnosis
Risk of malignancy
Cytological Techniques
Reviews
030209 endocrinology & metabolism
Review
Computational biology
DNA sequencing
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
cytopathology
Neoplasms
Cytology
Humans
Medicine
next generation sequencing
business.industry
biomarkers
High-Throughput Nucleotide Sequencing
General Medicine
molecular cytopathology
FNA
Cytopathology
030220 oncology & carcinogenesis
biomarker
business
Subjects
Details
- ISSN :
- 13652303 and 09565507
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Cytopathology
- Accession number :
- edsair.doi.dedup.....b5b7600cc313325c216bd51d42ebb946