Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells

Made available in DSpace on 2013-09-27T14:54:24Z (GMT). No. of bitstreams: 1 WOS000306286300025.pdf: 1836527 bytes, checksum: 38fbcddd13ac418aa35ffbd9378d21c0 (MD5) Previous issue date: 2012-07-01 Made available in DSpace on 2013-09-30T18:34:33Z (GMT). No. of bitstreams: 1 WOS000306286300025.pdf: 1836527 bytes, checksum: 38fbcddd13ac418aa35ffbd9378d21c0 (MD5) Previous issue date: 2012-07-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:48Z No. of bitstreams: 1 WOS000306286300025.pdf: 1836527 bytes, checksum: 38fbcddd13ac418aa35ffbd9378d21c0 (MD5) Made available in DSpace on 2014-05-20T14:04:48Z (GMT). No. of bitstreams: 1 WOS000306286300025.pdf: 1836527 bytes, checksum: 38fbcddd13ac418aa35ffbd9378d21c0 (MD5) Previous issue date: 2012-07-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective. This study evaluated the effects of photodynamic therapy (PDT) on buccal candidiasis in mice and on the adherence of yeast to buccal epithelial cells (BECs) in vitro.Study Design. A total of 56 immunosuppressed mice with buccal candidiasis were subjected to PDT, consisting of treatment with erythrosine (400 mu mol/L) followed by exposure to a green LED (14.34 J cm(-2)). After treatment, the yeasts recovered from the mice were quantified (CFU/mL) and analyzed for the effects of PDT on their adherence to BECs. The data were analyzed using ANOVA, the Tukey test, Kruskal-Wallis test and Student t test.Results. PDT significantly reduced the amount of yeast present in the lesions by 0.73 log(10) (P = .018) and reduced C. albicans adherence to BECs by 35% without damaging adjacent tissues (P = .045).Conclusions. Photodynamic therapy exhibited antifungal effects against C. albicans biofilms formed in vivo and reduced the capacity of C. albicans to adhere to BECs in vitro. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114:67-74) UNESP Univ Estadual Paulista, Dept Biosci & Oral Diag, Sch Dent Sao Jose dos Campos, Sao Jose Dos Campos, SP, Brazil UNESP Univ Estadual Paulista, Dept Biosci & Oral Diag, Sch Dent Sao Jose dos Campos, Sao Jose Dos Campos, SP, Brazil FAPESP: 09/52048-1 FAPESP: 10/18753-7 FAPESP: 09/12005-1 FAPESP: 10/01988-1 FAPESP: 10/00879-4