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Histological Method to Study the Effect of Shear Stress on Cell Proliferation and Tissue Morphology in a Bioreactor
- Source :
- Journal of Tissue Engineering and Regenerative Medicine, Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2019, ⟨10.1007/s13770-019-00181-3⟩
- Publication Year :
- 2019
- Publisher :
- Springer Singapore, 2019.
-
Abstract
- BACKGROUND: Tissue engineering represents a promising approach for the production of bone substitutes. The use of perfusion bioreactors for the culture of bone-forming cells on a three-dimensional porous scaffold resolves mass transport limitations and provides mechanical stimuli. Despite the recent and important development of bioreactors for tissue engineering, the underlying mechanisms leading to the production of bone substitutes remain poorly understood. METHODS: In order to study cell proliferation in a perfusion bioreactor, we propose a simplified experimental set-up using an impermeable scaffold model made of 2 mm diameter glass beads on which mechanosensitive cells, NIH-3T3 fibroblasts are cultured for up to 3 weeks under 10 mL/min culture medium flow. A methodology combining histological procedure, image analysis and analytical calculations allows the description and quantification of cell proliferation and tissue production in relation to the mean wall shear stress within the bioreactor. RESULTS: Results show a massive expansion of the cell phase after 3 weeks in bioreactor compared to static control. A scenario of cell proliferation within the three-dimensional bioreactor porosity over the 3 weeks of culture is proposed pointing out the essential role of the contact points between adjacent beads. Calculations indicate that the mean wall shear stress experienced by the cells changes with culture time, from about 50 mPa at the beginning of the experiment to about 100 mPa after 3 weeks. CONCLUSION: We anticipate that our results will help the development and calibration of predictive models, which rely on estimates and morphological description of cell proliferation under shear stress.
- Subjects :
- Scaffold
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
[SDV]Life Sciences [q-bio]
0206 medical engineering
Biomedical Engineering
Medicine (miscellaneous)
02 engineering and technology
03 medical and health sciences
Mice
Bioreactors
Tissue engineering
Shear stress
Bioreactor
Animals
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Cell Proliferation
Cell Size
0303 health sciences
Tissue Engineering
Chemistry
Cell growth
Histological Procedure
Histological Techniques
Biobehavioral Sciences
020601 biomedical engineering
Perfusion
Bone Substitutes
Hydrodynamics
NIH 3T3 Cells
Mechanosensitive channels
[SDV.IB]Life Sciences [q-bio]/Bioengineering
Original Article
Stress, Mechanical
Stem cell
Porosity
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 19327005 and 19326254
- Database :
- OpenAIRE
- Journal :
- Journal of Tissue Engineering and Regenerative Medicine, Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2019, ⟨10.1007/s13770-019-00181-3⟩
- Accession number :
- edsair.doi.dedup.....b6153266a5b1ed936ae49bfd24b8bffa
- Full Text :
- https://doi.org/10.1007/s13770-019-00181-3⟩