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Differential innate responses of chickens and ducks to low-pathogenic avian influenza
- Source :
- Avian Pathology 41 (2012) 6, Avian Pathology, 41(6), 519-529
- Publication Year :
- 2012
- Publisher :
- Informa UK Limited, 2012.
-
Abstract
- Ducks and chickens are hosts of avian influenza virus, each with distinctive responses to infection. To understand these differences, we characterized the innate immune response to low pathogenicity avian influenza virus H7N1 infection in chickens and ducks. Viral RNA was detected in the lungs of chickens from day 0.8 to 7, in ducks mainly at day 4. In both species viral RNA was detected in the bursa and gut. Infection in chickens resulted in up-regulation of interferon (IFN)-a and IFN-ß mRNA, while in the ducks IFN-¿ mRNA was strongly up-regulated in lung and bursa. In chickens and ducks all investigated pathogen recognition receptors (PRR) mRNA were up regulated, however, in the chicken lung Toll-like receptor (TLR)7 and melanoma differentiation-associated protein (MDA)-5 mRNA were strongly induced. TLR3, TLR7 and MDA-5 responses correlated with IFN-a and IFN-ß responses in chickens, but in ducks a correlation between IFN-a and TLR7, retinoic acid-inducible gene-I and MDA-5 was absent. We studied the responses of duck and chicken splenocytes to poly (I:C) and R848 analogues to analyze the regulation of PRRs without the interfering mechanisms of the influenza virus. This revealed IFN-a and IFN- ¿ responses in both species. MDA-5 was only strongly up-regulated in chicken splenocytes. In chickens splenocytes, time related PRR responses correlated with the IFN-a and IFN-ß response. In duck splenocytes this correlation was absent. In conclusion, chicken and duck differ in induction of MDA-5, TLR7 and IFN-a mRNA after an influenza virus infection in vivo and after in vitro stimulation with TLR antagonists.
- Subjects :
- viruses
Weight Gain
medicine.disease_cause
target-cells
Food Animals
Interferon
Influenza A virus
Lung
RIG-I
Bacteriologie
Toll-Like Receptors
virus diseases
Bacteriology, Host Pathogen Interaction & Diagnostics
Virology & Molecular Biology
toll-like receptor-7
Ducks
Cytokines
Influenza A Virus, H7N1 Subtype
RNA, Viral
recognition
medicine.drug
replication
animal structures
Enzyme-Linked Immunosorbent Assay
Biology
Virus
Species Specificity
Immunity
medicine
Animals
RNA, Messenger
h5n1
h9n2
Host Pathogen Interaction & Diagnostics
Innate immune system
General Immunology and Microbiology
rig-i
Bacteriology
gene-expression
Virology
Host Pathogen Interactie & Diagnostiek
Immunity, Innate
Influenza A virus subtype H5N1
Virologie & Moleculaire Biologie
ifn-beta
Influenza in Birds
Bacteriologie, Host Pathogen Interactie & Diagnostiek
TLR3
WIAS
a virus-infection
Animal Science and Zoology
Interferons
Chickens
Subjects
Details
- ISSN :
- 14653338 and 03079457
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Avian Pathology
- Accession number :
- edsair.doi.dedup.....b622bfd59e412a5357586f735eca6da5
- Full Text :
- https://doi.org/10.1080/03079457.2012.732691