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Comparative Study for the Determination of Fat-Soluble Vitamins in Rice Cereal Baby Foods Using HPLC-DAD and UHPLC-APCI-MS/MS
- Source :
- Foods, Vol 10, Iss 648, p 648 (2021), Foods, Volume 10, Issue 3
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Two liquid chromatographic systems, one coupled to atmospheric pressure chemical ionization and tandem mass spectrometric methods (UHPLC-APCI-MS/MS) and the other a high-performance liquid chromatographic coupled to diode array detector (HPLC-DAD) were used to develop and validate methods for the simultaneous determination of fat-soluble vitamins A, D3 and E in rice cereal baby foods. The chromatographic separation was performed on C18 columns with a mixture of methanol-acetonitrile as mobile phase for all methods. The extraction of fat-soluble vitamins included enzymatic hydrolysis with α-amylase, saponification, extraction with petroleum ether or n-hexane and purification with silica cartridge (only for vitamin D3). Quantification of vitamin D3 and E through UHPLC-APCI-MS/MS was performed by the use of internal standards (IS) D3-d3 and E-d6, respectively, while IS was not used for vitamin A. The methods were optimized and validated in terms of linearity, precision, trueness, limits of detection and quantification. The recoveries were in the range of 85.0–107% for retinol, 92.0–105% for α-tocopherol and 95.2–106% for cholecalciferol and the %RSD (Relative Standard Deviation) values ranged from 6.4% to 15%. The evaluation of the methods was also conducted through the estimation of uncertainties, the application in commercial samples and the participation in a proficiency test
- Subjects :
- Vitamin
Health (social science)
LC-APCI-MS/MS
Atmospheric-pressure chemical ionization
Plant Science
HPLC-DAD
lcsh:Chemical technology
Health Professions (miscellaneous)
Microbiology
Article
chemistry.chemical_compound
Chromatography detector
lcsh:TP1-1185
Petroleum ether
Rice cereal
Detection limit
Chromatography
food analysis
Extraction (chemistry)
enzymatic hydrolysis
baby foods
Fat-Soluble Vitamin
chemistry
extraction
saponification
estimation of uncertainty
fat-soluble vitamins
Food Science
Subjects
Details
- ISSN :
- 23048158
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Foods
- Accession number :
- edsair.doi.dedup.....b6286733176dff5a8e9ee22c0e3cc9d9