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Evaluation of the RIDA®GENE RT-PCR assays for detection of sapovirus, astrovirus, adenovirus, and rotavirus in stool samples of adults in Switzerland
- Publication Year :
- 2020
-
Abstract
- Sapovirus (SaV) and astrovirus (AstV) increasingly are recognized as cause of acute viral gastroenteritis (AGE). We evaluated the real-time RT-PCR assays RIDA®GENE SaV and viral stool panel II (RGN RT-PCR) for detection of SaV, AstV, adenovirus (AdV) F40/41 and rotavirus (RoV) in clinical stool samples (n = 69). Results were compared with reference singleplex RT-PCRs. The sensitivity for SaV, AstV and RoV are 100%, the specificity ranges from 98.1% to 100%. In 10 out of 11 AdV (all types) samples, the RGN RT-PCR for AdV F40/41 displayed negative results. Retrospectively, 196 stool specimens from adult patients previously tested negative for norovirus (NoV) were analyzed. In about 10% of NoV-negative stool samples, AdV (n = 9), RoV (n = 6), AstV (n = 3) or SaV (n = 3) were found. The RGN RT-PCR assays are useful for detection of enteric viruses other than NoV. This study emphasizes the need for further testing of NoV-negative stool samples in patients with AGE.
- Subjects :
- Adult
Male
Rotavirus
0301 basic medicine
Microbiology (medical)
10028 Institute of Medical Virology
viruses
030106 microbiology
610 Medicine & health
Real-Time Polymerase Chain Reaction
medicine.disease_cause
Sensitivity and Specificity
Sapovirus
2726 Microbiology (medical)
Adenoviridae
Astrovirus
Feces
03 medical and health sciences
fluids and secretions
0302 clinical medicine
medicine
Humans
In patient
030212 general & internal medicine
Gene
biology
Adult patients
business.industry
Reproducibility of Results
virus diseases
General Medicine
2725 Infectious Diseases
biology.organism_classification
Virology
Gastroenteritis
Infectious Diseases
Real-time polymerase chain reaction
10036 Medical Clinic
Norovirus
Female
business
Switzerland
Mamastrovirus
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....b633ec399095c16baafd48d300a2265c