Follicular fluid steroid profile in sows: Relationship to follicle size and oocyte quality

Identification of reliable characteristics of follicle quality and developmental competence has been pursued in numerous studies, but with inconsistent outcomes. Here, we aimed to identify these characteristics by analysis of the follicular fluid (FF) steroid profile in relation to cumulus-oocyte complex (COC) morphology and follicle size, followed by molecular substantiation. Multiparous sows at weaning were used to facilitate analysis at the start of the follicular phase of the oestrus cycle. Sows with a higher average follicle size (≥5 mm vs.
Sows with a higher percentage high-quality COCs have a distinct follicular fluid steroid profile and concomitant granulosa cell transcriptome.