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Microwave assisted solvent-free mannich bases: synthesis, characterization and effects of these compounds on HCA I and HCA II isozymes
- Source :
- Macedonian Journal of Chemistry and Chemical Engineering, Vol 40, Iss 1, Pp 51-56 (2021)
- Publication Year :
- 2021
- Publisher :
- Society of Chemists and Technologists of Macedonia, 2021.
-
Abstract
- Mannich bases (2a–d) of aromatic amines were synthesized by using a conventional microwave technique under solvent-free conditions and characterized by IR and NMR (1H and 13C) and elemental analysis. The inhibitory effects of the synthesized Mannich bases were examined in vitro by using hydratase and esterase assays on carbonic anhydrase I and II isozymes (hCA, EC 4.2.1.1) purified from human erythrocyte cells. Acetazolamide was used as the control compound. The values of IC50, the half-maximum inhibitory concentration, were found for hydratase and esterase activities. Only two compounds (2b and 2d) exhibited poor hCA I and hCA II inhibition effects on esterase activity. In contrast, compounds 2a and 2c could be used as carbonic anhydrase activators as a result of the increased esterase activity of hCA I and hCA II isozymes. © 2021, Macedonian Journal of Chemistry and Chemical Engineering. All Rights Reserved.
- Subjects :
- biology
Chemistry
Activator (genetics)
Stereochemistry
General Chemical Engineering
Glaucoma
General Chemistry
QD415-436
Isozyme
Esterase
Microwave assisted
Biochemistry
In vitro
activator, carbonic anhydrase, glaucoma, isozyme
Carbonic Anhydrase
Chemical engineering
Carbonic anhydrase
medicine
biology.protein
Activator
TP155-156
Carbonic Anhydrase I
Acetazolamide
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 18575625 and 18575552
- Volume :
- 40
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Macedonian Journal of Chemistry and Chemical Engineering
- Accession number :
- edsair.doi.dedup.....b675e78d7eff54fa90502e46c9b17881