Effect of the RW-Cb and its active ingredient like P-acid and P-aldehyde on primary rat osteoblasts

Ethnopharmacological relevance RW- Cb , the first processed product of Cibotium barometz (L.) J. Sm. (Dicksoniaceae) in TCM has been widely used to treat osteoporosis, a major worldwide health problem, influencing more and more people in the word et al. To do research on RW- Cb , one processed product of Cibotium barometz (L.) J. Sm. (Dicksoniaceae) in TCM has been widely used to treat osteoporosis, a major worldwide health problem, influencing more and more people in the word et al. Materials and methods The raw rhizome slices of Cibotium barometz were identified and both P -acid and P -aldehyde had a high purity. Primary rat osteoblasts were cultured and identified to be used for the assay. Results RW -Cb contributed the most to osteoblasts proliferation and the AKP activity among all the groups; osteoblasts proliferation effect of P -acid and P -aldehyde: the mixed group(high>middle>low)>the P -acid group (high>middle>low) or the P -aldehyde group(high>middle>low), P P -acid's high group was higher than P -aldehyde's high, the mixed of low and middle groups had no significant difference, the rest of groups' results were similar to the osteoblasts' proliferation, P Conclusions RW -Cb and its index constituents like P -acid and P -aldehyde could promote proliferation and differentiation of primary rat osteoblasts cultured in vitro, and it could be the theoretical basis for the treatment of osteoporosis.