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Immunomodulatory effect of γ-irradiatedAstragaluspolysaccharides on immunosuppressed broilers
- Source :
- Animal Science Journal. 90:117-127
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- In this study, we irradiated Astragalus polysaccharides (APS) using 25 kGy 60 Co γ ray to obtain γ-irradiated Astragalus polysaccharides (IAPS) and then investigated the effects of IAPS on growth performance and immune function of cyclophosphamide (CPM)-treated broilers. The physicochemical properties of APS and IAPS (molecular weight, water solubility, viscosity, morphological and structural properties) were evaluated. Then, 384 one-day-old Arbor Acres broiler chicks with similar initial weight were randomly assigned into 6 groups: the non-treated group (control), and CPM-treated groups were fed either a basal diet or the diets containing 900 mg/kg APS, or 900, 600, 300 mg/kg IAPS, respectively. On days 16, 18, and 20, all broilers except for the control group were intramuscularly injected with 0.5 ml CPM (40 mg/kg·BW). Broilers in the control group were intramuscularly injected with 0.5 ml sterilized saline (0.75%, wt/vol). This trial lasted for 21 days. The physicochemical treatment showed that γ irradiation could decrease the molecular weight and viscosity, and increase the water solubility of APS (p < 0.05), whereas the structural properties of APS was not affected. In the animal trial, 900 mg/kg APS or 900, 600 mg/kg IAPS relieved the decreased growth performance, thymus index, T lymphocytes proliferation, serum IgG concentration, NOS activity and the increased blood heterophil:lymphocyte ratio in CPM-treated broilers (p < 0.05). CPM-induced decreases in B lymphocytes proliferation and serum IgM concentration were only increased by IAPS at 900 mg/kg (p < 0.05). Overall, both APS and IAPS alleviated CPM-induced immunosuppression. Especially, IAPS possessed better immunomodulatory effect than APS, indicating that γ irradiation could be used as an effective method to enhance the immunomodulatory activity of APS.
- Subjects :
- Chemical Phenomena
Cyclophosphamide
T-Lymphocytes
Lymphocyte
medicine.medical_treatment
Thymus Gland
02 engineering and technology
Injections, Intramuscular
Animal science
Immune system
Polysaccharides
Immune Tolerance
medicine
Animals
Immunologic Factors
Irradiation
Saline
Viscosity
Chemistry
0402 animal and dairy science
Broiler
04 agricultural and veterinary sciences
General Medicine
Astragalus propinquus
021001 nanoscience & nanotechnology
Animal Feed
040201 dairy & animal science
Diet
Molecular Weight
medicine.anatomical_structure
Immunoglobulin M
Solubility
Astragalus polysaccharide
Gamma Rays
Dietary Supplements
Food Irradiation
0210 nano-technology
General Agricultural and Biological Sciences
Chickens
Immunosuppressive Agents
Decreased growth
Drugs, Chinese Herbal
medicine.drug
Subjects
Details
- ISSN :
- 13443941
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- Animal Science Journal
- Accession number :
- edsair.doi.dedup.....b6b0edef43968f3289dcefaac20cf2ee