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TNF alpha inhibits Schwann cell proliferation, connexin46 expression, and gap junctional communication
- Source :
- Molecular and cellular neurosciences. 7(6)
- Publication Year :
- 1996
-
Abstract
- Schwann cell responses to nerve injury are stimulated, in part, by inflammatory cytokines. This study compares changes in the phenotype of cultured Schwann cells after exposure to the cytokine tumor necrosis factor (TNF)-alpha or the mitogen neu differentiation factor (NDF)-beta. TNF alpha inhibited proliferation in a dose-dependent manner without altering Schwann cell survival. TNF alpha also reduced both gap junctional conductance and Lucifer yellow dye coupling between Schwann cells. Moreover, both Po and glial fibrillary acidic protein (GFAP) immunoreactivity were reduced. By contrast, NDF beta initially had little effect on cell division although it reduced junctional coupling within 8 h. However, by 48 h, NDF beta stimulated proliferation with a concomitant increase in coupling. Dividing Schwann cells (BrdU+) were preferentially dye coupled compared to nondividing cells, indicating an association between proliferation and coupling. Moreover, cultured Schwann cells expressed connexin46 mRNA and protein, and changes in the levels of the protein correlated with the degree of proliferation and coupling. The data thus provide evidence for cytokine-induced modulation of Schwann cell antigenic phenotype, proliferation, and gap junction properties. These observations suggest that enhanced gap junctional communication among Schwann cells after nerve injury could help to coordinate cellular responses to the injury, and that TNF alpha may be a signal which terminates proliferation as well as junctional communication.
- Subjects :
- Cell division
medicine.medical_treatment
Schwann cell
Nerve Tissue Proteins
Cell Communication
Polymerase Chain Reaction
Connexins
Schwann cell proliferation
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Glial Fibrillary Acidic Protein
medicine
Animals
RNA, Messenger
Molecular Biology
Cells, Cultured
Lucifer yellow
Glial fibrillary acidic protein
biology
Tumor Necrosis Factor-alpha
Gap junction
Gap Junctions
Cell Biology
Sciatic Nerve
Cell biology
Rats
medicine.anatomical_structure
Cytokine
nervous system
chemistry
Animals, Newborn
Gene Expression Regulation
biology.protein
Tumor necrosis factor alpha
Schwann Cells
Myelin P0 Protein
Cell Division
Subjects
Details
- ISSN :
- 10447431
- Volume :
- 7
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular neurosciences
- Accession number :
- edsair.doi.dedup.....b6ca40e5cfb0ffacff56657588ccdeed