A universal signal-on electrochemical assay for rapid on-site quantitation of vibrio parahaemolyticus using aptamer modified magnetic metal-organic framework and phenylboronic acid-ferrocene co-immobilized nanolabel

Sensitive and rapid detection of pathogenic bacteria remains important and challenging for food safety and preventing outbreaks of foodborne disease. The major limitations of standard analytical methods for detecting vibrio parahaemolyticus (V.P) lie in their bulky equipment and tedious and long-time operation. This study presents an electrochemical aptasensor for the rapid on-site quantification of V.P in seafood. Magnetic nanoscale metal-organic frameworks (Fe3O4@NMOF) labeled with an aptamer against V.P served as capture probes, while gold nanoparticles combined with phenylboronic acid and ferrocene acted as the nanolabels. When detecting V.P, the sandwich-type complex of capture probe–V.P–nanolabel was formed and magnetically attached to a screen-printed electrode (SPE) for signal measurement. Under optimal conditions, the increase in the ferrocene electrochemical signals could assess the V.P amount; the quantified concentration range was 10–109 cfu/mL. Then, the developed signal-on sensor successfully detected V.P in real seafood samples, exhibiting many advantages. It could not only specifically enrich and rapidly separate the V.P in complex samples but also largely amplify the signal. Moreover, using compact SPE with a detection time of maximum 20 min as the measurement platform allows rapid on-site assays. Thus, the proposed method is a feasible strategy for screening V.P in seafood.