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Directs effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on adaptive immunogenesis
- Source :
- Hum Vaccin Immunother
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- Background: We studied direct effects of human granulocyte-macrophage colony stimulating factor (GM-CSF) on phenotypical characteristics and cytokine-production of non-activated and activated human monocytes/macrophages (Mc/Mphs) and T cells. Methods: Purified Mc/Mphs were activated by bacterial lipopolysaccharide (LPS, 1 μg/ml) for 24 h, while T cells were activated by particles conjugated and antibodies (Abs) against human CD2, CD3, and CD28 for 48 h. Results: GM-CSF treatment (0.01–10 ng/ml) was shown to reduce percentages of CD197 (CCR7)-positive cells in non-activated Mph cultures, without affecting significantly CD14(+) (LPS co-receptor), CD16(+) (FcγRIII, low-affinity Fc-receptor), CD119(+) (interferon-gamma receptor 1), and CD124(+) (IL4 receptor α-subunit) cells. In addition, GM-CSF reduced relative numbers of CD197(+) cells, as well as CD14(+), CD16(+), and CD119(+) cells in activated Mph cultures without affecting CD124(+) cell distribution. GM-CSF at the highest dose of 10 ng/ml enhanced TNF-α and IL-6 (but not IL-1β and IL-10) production in activated Mc/Mphs. In activated T cell cultures, GM-CSF at 0.1–1.0 ng/ml augmented CD38(+) cell numbers in naïve СD45RA(+)/СD197(+) and central memory СD45RA(−)/СD197(+) cell subsets, with no effect on effector СD45RA(−)/СD197(−) and terminally differentiated effector СD45RA(+)/СD197(−) cells. GM-CSF at a low dose (0.01 ng/ml) down-regulated INF-γ production, while at a high dosage (10.0 ng/ml) up-regulated IL-2 and IL-4 production. Conclusion: In general, the results suggest that GM-CSF is able to facilitate the implication of both Mph and T cells in the adaptive immunogenesis.
- Subjects :
- Lipopolysaccharides
T-Lymphocytes
030231 tropical medicine
Immunology
Adaptive Immunity
Biology
Lymphocyte Activation
Monocytes
03 medical and health sciences
0302 clinical medicine
medicine
Humans
Immunology and Allergy
Monocytes macrophages
030212 general & internal medicine
Cells, Cultured
Pharmacology
Macrophages
Direct effects
Granulocyte-Macrophage Colony-Stimulating Factor
Colony-stimulating factor
Phenotype
Granulocyte macrophage colony-stimulating factor
Cytokines
Research Paper
medicine.drug
Subjects
Details
- ISSN :
- 2164554X and 21645515
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Human Vaccines & Immunotherapeutics
- Accession number :
- edsair.doi.dedup.....b6e3e9bcf2d5c6193bc36c723b6088ba