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EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS
- Source :
- Arquivos Brasileiros de Cirurgia Digestiva : ABCD, ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo), Volume: 30, Issue: 2, Pages: 132-138, Published: JUN 2017, ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo), Volume: 31, Issue: 3, Article number: e1391, Published: 16 AUG 2018, ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) v.30 n.2 2017, ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo), Colégio Brasileiro de Cirurgia Digestiva (CBCD), instacron:CBCD, ABCD: Arquivos Brasileiros de Cirurgia Digestiva, Vol 30, Iss 2, Pp 132-138 (2017)
- Publication Year :
- 2016
-
Abstract
- Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p
- Subjects :
- Mucina-2
Male
medicine.medical_specialty
RD1-811
Colon
medicine.medical_treatment
Sucralfate
Enema
RC799-869
Mucin 2
Gastroenterology
Sucralfato
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
Animals
Fatty acids volatile
Derivation
Colitis
Intestinal Mucosa
Rats, Wistar
Cólon
Saline
Feces
Diversion colitis
Mucin-2
business.industry
General Medicine
Diseases of the digestive system. Gastroenterology
medicine.disease
Rats
030220 oncology & carcinogenesis
Surgery
030211 gastroenterology & hepatology
Colite
Ácidos graxos voláteis
Original Article
business
medicine.drug
Subjects
Details
- ISSN :
- 23176326
- Volume :
- 30
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery
- Accession number :
- edsair.doi.dedup.....b6f92df4c86f8f717c857183aebc8716