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Erythropoietin suppresses peritoneal fibrosis in rat experimental model
- Source :
- European journal of pharmacology. 604(1-3)
- Publication Year :
- 2008
-
Abstract
- Peritoneal dialysis (PD) is an alternative treatment of patients with end-stage renal disease. Unfortunately, long term peritoneal dialysis causes injury of the peritoneum associated with ultra filtration failure. Erythropoietin (EPO) is a potent stimulator of elytroid progenitor cells and its expression is enhanced by hypoxia. The aim of the present study was to evaluate the effect of EPO on the development of experimental peritoneal fibrosis induced by chlorhexidine gluconate (CG). A peritoneal fibrosis model was established using rats treated intraperitoneally with injection of CG. EPO was administered at the dose of 5000 U/kg i.p. three time per week for three weeks. When compared to CG-treated rats, EPO (5000 U/kg i.p. three time per week for three weeks) treated rats subjected to GC-induced peritoneal fibrosis experienced a significantly lower rate in the extent and severity of the histological signs of peritoneal injury. EPO also caused a substantial reduction of (i) the rise in myeloperoxidase activity (mucosa), (ii) the expression in the tissue of TNF-alpha, TGFbeta and VEGF (iii) the increase in staining (immunohistochemistry) for nitrotyrosine and for PAR, as well as (iv) the NF-kappaB activation caused by CG in the peritoneum. Thus, EPO treatment reduces the degree of peritoneal fibrosis caused by CG. We propose that this evidence may help to clarify the potential therapeutic actions of EPO in patients with peritoneal fibrosis.
- Subjects :
- medicine.medical_specialty
medicine.medical_treatment
Blotting, Western
Urology
Peritoneal Diseases
Thiobarbituric Acid Reactive Substances
Peritoneal dialysis
chemistry.chemical_compound
Peritoneum
chlorhexidine gluconate
Fibrosis
medicine
Animals
Progenitor cell
Erythropoietin
fibrosis
myeloperoxidase activity
TNF-alpha
TGFbeta
VEGF
Peritoneal Fibrosis
Pharmacology
business.industry
Nitrotyrosine
Chlorhexidine
Rats, Inbred Strains
Hypoxia (medical)
medicine.disease
Rats
Disease Models, Animal
medicine.anatomical_structure
chemistry
Immunology
medicine.symptom
business
Peritoneal Dialysis
medicine.drug
Subjects
Details
- ISSN :
- 18790712
- Volume :
- 604
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- European journal of pharmacology
- Accession number :
- edsair.doi.dedup.....b70203dce35144dcf8bb5dfa6fef6d82