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Circulating microparticles, protein C, free protein S and endothelial vascular markers in children with sickle cell anaemia
- Source :
- Journal of Extracellular Vesicles, Vol 4, Iss 0, Pp 1-15 (2015), Journal of Extracellular Vesicles, Journal of Extracellular Vesicles; Vol 4 (2015) incl supplements
- Publication Year :
- 2015
- Publisher :
- Taylor & Francis Group, 2015.
-
Abstract
- Introduction: Circulating microparticles (MP) have been described in sickle cell anaemia (SCA); however, their interaction with endothelial markers remains unclear. We investigated the relationship between MP, protein C (PC), free protein S (PS), nitric oxide (NO), endothelin-1 (ET-1) and adrenomedullin (ADM) in a large cohort of paediatric patients.Method: A total of 111 children of African ethnicity with SCA: 51 in steady state; 15 in crises; 30 on hydroxyurea (HU) therapy; 15 on transfusion; 17 controls (HbAA) of similar age/ethnicity. MP were analysed by flow cytometry using: Annexin V (AV), CD61, CD42a, CD62P, CD235a, CD14, CD142 (tissue factor), CD201 (endothelial PC receptor), CD62E, CD36 (TSP-1), CD47 (TSP-1 receptor), CD31 (PECAM), CD144 (VE-cadherin). Protein C, free PS, NO, pro-ADM and C-terminal ET-1 were also measured.Results: Total MP AV was lower in crisis (1.26×106 ml−1; 0.56–2.44×106) and steady state (1.35×106 ml−1; 0.71–3.0×106) compared to transfusion (4.33×106 ml−1; 1.6–9.2×106, p0.9, p
- Subjects :
- medicine.medical_specialty
Histology
CD36
sickle cell anaemia
protein C
Protein S
nitric oxide
Internal medicine
medicine
Platelet
Platelet activation
Original Research Article
lcsh:QH573-671
microparticles
Sickle Cell Anaemia, microparticles, protein C, protein S, adrenomedullin, endothelin-1 , nitric oxide
Red Cell
biology
business.industry
lcsh:Cytology
Cell Biology
Endothelin 1
Adrenomedullin
Endocrinology
Immunology
adrenomedullin
endothelin-1
biology.protein
business
protein S
Protein C
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20013078
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of Extracellular Vesicles
- Accession number :
- edsair.doi.dedup.....b70333dd66f13d58d9166c289e9b423e