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Identification and Initial Characterization of Prophages in Vibrio campbellii
- Source :
- PLoS ONE, Vol 11, Iss 5, p e0156010 (2016), PLoS One, PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- Phages are bacteria targeting viruses and represent the most abundant biological entities on earth. Marine environments are exceptionally rich in bacteriophages, harboring a total of 4x10(30) viruses. Nevertheless, marine phages remain poorly characterized. Here we describe the identification of intact prophage sequences in the genome of the marine gamma-proteobacterium Vibrio campbellii ATCC BAA-1116 (formerly known as V. harveyi ATCC BAA-1116), which presumably belong to the family of Myoviridae. One prophage was found on chromosome I and shows significant similarities to the previously identified phage Phi HAP-1. The second prophage region is located on chromosome II and is related to Vibrio phage kappa. Exposure of V. campbellii to mitomycin C induced the lytic cycle of two morphologically distinct phages and, as expected, extracellular DNA from induced cultures was found to be specifically enriched for the sequences previously identified as prophage regions. Heat stress (50 degrees C, 30 min) was also found to induce phage release in V. campbellii. Notably, promoter activity of two representative phage genes indicated heterogeneous phage induction within the population.
- Subjects :
- 0301 basic medicine
Hot Temperature
Physiology
Prophages
viruses
lcsh:Medicine
Viral Packaging
Bacteriophage
Fluorescence Microscopy
Lysogen
Medicine and Health Sciences
Bacteriophages
Cloning, Molecular
lcsh:Science
Virus Release
Genetics
Viral Genomics
Microscopy
Multidisciplinary
biology
Chromosome Biology
Chromosome Mapping
Light Microscopy
Genomics
Chromosomes, Bacterial
Temperateness
Lytic cycle
Myoviridae
Viruses
Research Article
Lysis (Medicine)
Microbial Genomics
Research and Analysis Methods
Microbiology
Chromosomes
03 medical and health sciences
Caudovirales
Virology
Lysogenic cycle
Tissue Repair
Vibrio campbellii
Prophage
Vibrio
Base Sequence
Bacteria
lcsh:R
Organisms
Biology and Life Sciences
Computational Biology
Sequence Analysis, DNA
Cell Biology
Genome Analysis
Genomic Libraries
biology.organism_classification
Viral Replication
030104 developmental biology
lcsh:Q
Physiological Processes
Genome, Bacterial
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....b754f1193761b5f5443839745605d69a