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Acceptor specificity in the transglycosylation reaction using Endo-M
- Source :
- Carbohydrate Research. 345:2458-2463
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- To determine the structural specificity of the glycosyl acceptor of the transglycosylation reaction using endo-β-N-acetylglucosaminidase (ENGase) (EC 3.2.1.96) from Mucor hiemalis (Endo-M), several acceptor derivatives were designed and synthesized. The narrow regions of the 1,3-diol structure from the 4- to 6-hydroxy functions of GlcNAc were found to be essential for the transglycosylation reaction using Endo-M. Furthermore, it was determined that Endo-M strictly recognizes a 1,3-diol structure consisting of primary and secondary hydroxyl groups.
- Subjects :
- Glycosylation
Stereochemistry
Kinetics
Biochemistry
Substrate Specificity
Analytical Chemistry
chemistry.chemical_compound
Chromatography, High Pressure Liquid
Pyrans
chemistry.chemical_classification
Mucor
Chromatography, Reverse-Phase
biology
Organic Chemistry
Glycosyl acceptor
General Medicine
biology.organism_classification
Acceptor
Oxygen
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Enzyme
Mucor hiemalis
chemistry
Substrate specificity
Subjects
Details
- ISSN :
- 00086215
- Volume :
- 345
- Database :
- OpenAIRE
- Journal :
- Carbohydrate Research
- Accession number :
- edsair.doi.dedup.....b75baa2a1801a697b25bc64567a758fa