Back to Search Start Over

Large-scale production of cellulose-binding domains. Adsorption studies using CBD-FITC conjugates

Large-scale production of cellulose-binding domains. Adsorption studies using CBD-FITC conjugates

Authors :
Joana Carvalho
Manuel Mota
Ricardo Pinto
Miguel Gama
Universidade do Minho
Source :
Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
Publication Year :
2006
Publisher :
Springer Science and Business Media LLC, 2006.

Abstract

A method for the gram-scale production of cellulose-binding domains (CBD) through the proteolytic digestion of a commercial nzymatic preparation (Celluclast) was developed. The CBD obtained, isolated from Trichoderma reesei cellobiohydrolase I, is highly pure and heavily glycosylated. The purified peptide has a molecular weight of 8.43 kDa, comprising the binding module, a part of the linker, and about 30% glycosidic moiety. Its properties may thus be different from recombinant ones expressed in bacteria. CBDfluorescein isothiocyanate conjugates were used to study the CBD-cellulose interaction. The presence of fluorescent peptides adsorbed on crystalline and amorphous cellulose fibers suggests that amorphous regions have a higher concentration of binding sites. The adsorption is reversible, but desorption is a very slow process.<br />Fundação para a Ciência e a Tecnologia (FCT)

Details

ISSN :
1572882X and 09690239
Volume :
13
Database :
OpenAIRE
Journal :
Cellulose
Accession number :
edsair.doi.dedup.....b7612fc13dc01a17b3d535888822736d
Full Text :
https://doi.org/10.1007/s10570-006-9060-5