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Large-scale production of cellulose-binding domains. Adsorption studies using CBD-FITC conjugates
Large-scale production of cellulose-binding domains. Adsorption studies using CBD-FITC conjugates
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- A method for the gram-scale production of cellulose-binding domains (CBD) through the proteolytic digestion of a commercial nzymatic preparation (Celluclast) was developed. The CBD obtained, isolated from Trichoderma reesei cellobiohydrolase I, is highly pure and heavily glycosylated. The purified peptide has a molecular weight of 8.43 kDa, comprising the binding module, a part of the linker, and about 30% glycosidic moiety. Its properties may thus be different from recombinant ones expressed in bacteria. CBDfluorescein isothiocyanate conjugates were used to study the CBD-cellulose interaction. The presence of fluorescent peptides adsorbed on crystalline and amorphous cellulose fibers suggests that amorphous regions have a higher concentration of binding sites. The adsorption is reversible, but desorption is a very slow process.<br />Fundação para a Ciência e a Tecnologia (FCT)
- Subjects :
- 0106 biological sciences
Materials science
Polymers and Plastics
Stereochemistry
FITC
Peptide
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
010608 biotechnology
Organic chemistry
Fluorescein isothiocyanate
Trichoderma reesei
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Science & Technology
biology
Proteolytic enzymes
Glycosidic bond
Cellulose binding
biology.organism_classification
3. Good health
chemistry
Cellulose-binding domains
Proteolysis
Isothiocyanate
Adsorption
Linker
Subjects
Details
- ISSN :
- 1572882X and 09690239
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Cellulose
- Accession number :
- edsair.doi.dedup.....b7612fc13dc01a17b3d535888822736d
- Full Text :
- https://doi.org/10.1007/s10570-006-9060-5