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Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations
- Source :
- Pathology International. 69:637-645
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- We previously characterized the morphological characteristics of malignant pleural mesothelioma (MPM) cells with 9p21 homozygous deletion (HD) using a combination of the virtual microscopy and fluorescence in situ hybridization (FISH). In this study, we investigated whether MPM cells with BRCA1-associated protein 1 (BAP1) loss show the same morphological characteristics identified in MPM cells with 9p21 HD. MPM cells with either BAP1 loss detected by immunocytochemistry (ICC) or 9p21 HD detected by FISH were identified via virtual microscopy prior to ICC or FISH, followed by analysis and quantification of their morphological characteristics. MPM cells with BAP1 loss or 9p21 HD exhibited significantly more frequent cell-in-cell engulfment, multinucleation, and larger multicellular clusters composed of more than 10 cells than reactive mesothelial cells. In conclusion, MPM cells with BAP1 loss or 9p21 HD share similar cytological features, indicating that the same morphological criteria can be used to detect MPM cells harboring such genetic aberrations.
- Subjects :
- Male
Mesothelioma
0301 basic medicine
Pathology
medicine.medical_specialty
Pleural Neoplasms
Reactive mesothelial cells
Immunocytochemistry
Biology
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Biomarkers, Tumor
medicine
Humans
Gene
Aged
Sequence Deletion
Aged, 80 and over
BAP1
medicine.diagnostic_test
Pleural mesothelioma
Tumor Suppressor Proteins
General Medicine
Middle Aged
Gene deletion
Immunohistochemistry
030104 developmental biology
Effusion
030220 oncology & carcinogenesis
Female
Chromosome Deletion
Chromosomes, Human, Pair 9
Ubiquitin Thiolesterase
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 14401827 and 13205463
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- Pathology International
- Accession number :
- edsair.doi.dedup.....b77c2ac86c414e0e5970e6f5fec502e8