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CD4+CD25+regulatory T cells in irritable bowel syndrome patients
- Source :
- Neurogastroenterology & Motility. 19:119-125
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- The aetiology of the irritable bowel syndrome (IBS) is incompletely understood. A low-grade colonic inflammation is frequently seen, but it is unclear to what extent this phenomenon contributes to the pathophysiology of IBS. CD4(+)CD25(+) regulatory T cells (Treg) are implicated to play an important role in suppressing intestinal inflammation. We, therefore, examined whether the intestinal inflammatory process in IBS patients is the result of an altered function and/or frequency of CD25(+) Treg cells. Patients with IBS (n = 34), fulfilling the Rome II criteria, were compared with controls (n = 26). The suppressive activity of blood CD25(+) Treg cells was determined and the frequency of colonic and blood CD25(+) Treg cells was analysed by flow cytometry. The expression of the Treg marker, FOXP3 mRNA, in colonic biopsies was determined by reverse transcription-polymerase chain reaction. Blood CD25(+) Treg cells from IBS patients suppressed the proliferation of blood CD4(+)CD25(low/-) T cells. Similar frequencies of CD25(+) Treg cells were recorded in mucosa and blood of IBS patients and controls. FOXP3 mRNA was equally expressed in the colonic mucosa of patients with IBS and controls. In conclusion, the low-grade intestinal inflammation recorded in patients with IBS is not associated with an altered function or frequency of CD25(+) Treg cells.
- Subjects :
- Adult
Male
medicine.medical_specialty
Colon
Physiology
Biopsy
Gene Expression
chemical and pharmacologic phenomena
Inflammation
T-Lymphocytes, Regulatory
Gastroenterology
Flow cytometry
Irritable Bowel Syndrome
Internal medicine
medicine
Humans
RNA, Messenger
IL-2 receptor
Intestinal Mucosa
Irritable bowel syndrome
Messenger RNA
medicine.diagnostic_test
Endocrine and Autonomic Systems
business.industry
Interleukin-2 Receptor alpha Subunit
FOXP3
Forkhead Transcription Factors
hemic and immune systems
Middle Aged
Colitis
Flow Cytometry
medicine.disease
Pathophysiology
CD4 Antigens
Immunology
Female
medicine.symptom
business
Biomarkers
Subjects
Details
- ISSN :
- 13652982 and 13501925
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Neurogastroenterology & Motility
- Accession number :
- edsair.doi.dedup.....b787a3d5ddfffdffa2cb2300578e9bd2