Back to Search
Start Over
Coordination of EZH2 and SOX2 specifies human neural fate decision
- Source :
- Cell Regeneration, Cell Regeneration, Vol 10, Iss 1, Pp 1-13 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Singapore, 2021.
-
Abstract
- Polycomb repressive complexes (PRCs) are essential in mouse gastrulation and specify neural ectoderm in human embryonic stem cells (hESCs), but the underlying molecular basis remains unclear. Here in this study, by employing an array of different approaches, such as gene knock-out, RNA-seq, ChIP-seq, et al., we uncover that EZH2, an important PRC factor, specifies the normal neural fate decision through repressing the competing meso/endoderm program. EZH2−/− hESCs show an aberrant re-activation of meso/endoderm genes during neural induction. At the molecular level, EZH2 represses meso/endoderm genes while SOX2 activates the neural genes to coordinately specify the normal neural fate. Moreover, EZH2 also supports the proliferation of human neural progenitor cells (NPCs) through repressing the aberrant expression of meso/endoderm program during culture. Together, our findings uncover the coordination of epigenetic regulators such as EZH2 and lineage factors like SOX2 in normal neural fate decision. Supplementary Information The online version contains supplementary material available at 10.1186/s13619-021-00092-6.
- Subjects :
- Medicine (General)
Neuroectoderm
QH301-705.5
fungi
Cell Biology
macromolecular substances
Biology
Embryonic stem cell
Neural stem cell
Cell biology
Gastrulation
R5-920
medicine.anatomical_structure
SOX2
embryonic structures
medicine
Biology (General)
Stem cell
Endoderm
Neural development
Developmental Biology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20459769
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Cell Regeneration
- Accession number :
- edsair.doi.dedup.....b79012f63e38cd7d2f266ee22374793d