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Characterization of GAB3 and its association with NNV resistance in the Asian seabass
- Source :
- Fish & Shellfish Immunology. 104:18-24
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Understanding the functions of genes related to disease resistance and identifying polymorphisms in these genes are essential in molecular breeding for disease resistance. Viral nervous necrosis (VNN) is one of the major diseases in the Asian seabass, Lates calcarifer. Our previous works on QTL mapping, GWAS and cell-line transcriptome analysis of the Asian seabass after NNV challenge revealed that the gene GAB3 might be a candidate gene for VNN resistance. In this study, we cloned and characterized GAB3, and identified SNPs in the gene of the Asian seabass. The cDNA of the gene was 2165 bp, containing an ORF of 1674 bp encoding 557 amino acids. The gene consisted of 10 exons and nine introns. It was ubiquitously expressed in normal fish. An analysis of the association between two SNPs in the second intron and NNV resistance in 1035 fish descended from 43 families revealed that the two SNPs were significantly associated with VNN resistance. After NNV infection, the expression of GAB3 was significantly increased in the brain, spleen, muscle and gut, and was suppressed in the liver. The GAB3 protein was localized in the nucleus. Overexpression of GAB3 with specific GAB3-pcDNA was positively correlated to increased viral RNA and titer in NNV-infected Asian seabass cells. Our study provides new evidence to support that GAB3 may be an important gene related to NNV resistance. In addition, the SNPs provide DNA markers for the selection of candidate genes resistance to NNV at the juvenile stage of Asian seabass.
- Subjects :
- Fish Proteins
0301 basic medicine
Candidate gene
Genome-wide association study
Single-nucleotide polymorphism
Aquatic Science
Biology
Transcriptome
Fish Diseases
03 medical and health sciences
Exon
RNA Virus Infections
Complementary DNA
Animals
Environmental Chemistry
Nodaviridae
Gene
Phylogeny
GRB2 Adaptor Protein
Genetics
Gene Expression Profiling
Intron
04 agricultural and veterinary sciences
General Medicine
Immunity, Innate
030104 developmental biology
Gene Expression Regulation
040102 fisheries
0401 agriculture, forestry, and fisheries
Bass
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....b7bf0711bc222214159e6b5aa0078d87