Back to Search
Start Over
Profiles of Physarum Microplasmodial Phosphatase Activity Crucial to Cytoplasmic Streaming and Spherule Formation
- Source :
- Cell biochemistry and biophysics. 77(4)
- Publication Year :
- 2019
-
Abstract
- This study aimed to investigate for the first time, the profile of Physarum microplasmodial phosphatase (PPH) activity toward the phosphorylated light chain of Physarum myosin II (PLCM) at pH 7.6, the velocity of cytoplasmic streaming, and PPH expression in spherule formation during dark starvation (DS). In this study, we cloned the full-length cDNA of PPH using polymerase chain reaction, based on the N-terminal amino acid sequence of the purified enzyme. The cDNA contained an open reading frame (ORF) of 1245 bp, corresponding to 415 amino acids. We confirmed that a rapid increase in PPH activity toward PLCM and a rapid decrease in cytoplasmic streaming velocity precede spherule formation by Physarum microplasmodia. The profiles of increase in PPH activity toward PLCM, PPH expression, and PPH accumulation during DS were correlated with spherule formation in the Physarum microplasmodia. Moreover, application of the wheat germ cell-free expression system resulted in the successful production of recombinant PPH and in the expression of phosphatase activity toward PLCM. These results suggest that PPH is involved in the cessation of cytoplasmic streaming in Physarum microplasmodia during DS.
- Subjects :
- 0301 basic medicine
Phosphatase
Biophysics
Protozoan Proteins
Cytoplasmic Streaming
Physarum polycephalum
Biochemistry
Physarum
03 medical and health sciences
Complementary DNA
Amino Acid Sequence
Cloning, Molecular
Phosphorylation
Peptide sequence
chemistry.chemical_classification
Myosin Type II
030102 biochemistry & molecular biology
biology
Chemistry
fungi
Cell Biology
General Medicine
biology.organism_classification
Phosphoric Monoester Hydrolases
Recombinant Proteins
Amino acid
Cytoplasmic streaming
Open reading frame
030104 developmental biology
Subjects
Details
- ISSN :
- 15590283
- Volume :
- 77
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cell biochemistry and biophysics
- Accession number :
- edsair.doi.dedup.....b7dacc16cd306d103758a05f31625e72