Back to Search
Start Over
Macrophage colony-stimulating factor (M-CSF) is an intermediate in the process of luteinizing hormone-induced decrease in natriuretic peptide receptor 2 (NPR2) and resumption of oocyte meiosis
- Source :
- Journal of Ovarian Research, Vol 10, Iss 1, Pp 1-8 (2017), Journal of Ovarian Research
- Publication Year :
- 2017
- Publisher :
- BMC, 2017.
-
Abstract
- Background Luteinizing hormone (LH) regulation of the ligand, natriuretic peptide precursor type C, and its receptor, natriuretic peptide receptor 2 (NPR2), is critical for oocyte maturation; however, the mechanism is not fully understood. Macrophage colony-stimulating factor (M-CSF) has recently been shown to be involved in oocyte maturation and ovulation. In the present study we determined whether or not M-CSF plays a role in the intermediate signal that mediates LH regulation of NPR2 in resumption of oocyte meiosis. Methods Immature female C57BL/6 mice were injected i.p. with 5 IU of equine chorionic gonadotropin (eCG) to stimulate follicle development. After 44–48 h, the eCG-stimulated mice were injected i.p. with an ovulatory dose of 5 IU of human chorionic gonadotropin (hCG). The ovaries were excised at selected times. Pre-ovulatory follicles (POFs) and cumulus-oocyte complexes were cultured in different media. Immunohistochemical and quantitative real-time PCR analyses were used to assess the expression of M-CSF, M-CSF receptor (M-CSF-R), and NPR2. The presence of germinal vesicle breakdown (GVBD) was examined under a stereomicroscope to morphologically evaluate resumption of oocyte meiosis. Results NPR2 was mainly expressed in cumulus cells of pre-ovulatory follicles, while M-CSF and M-CSF-R were expressed in both mural granulosa and cumulus cells. The levels of M-CSF/M-CSF-R and NPR2 decreased within 4 h after treatment of hCG. M-CSF not only reduced the expression of NPR2 mRNA via its receptor (M-CSF-R), but also increased the proportion of GVBD in oocytes. Conclusion M-CSF serves as an intermediate signal, thus inducing a vital decrease in the NPR2 levels in cumulus cells, and regulates the process of LH-induced resumption of meiosis.
- Subjects :
- 0301 basic medicine
Luteinizing hormone
medicine.medical_specialty
endocrine system
Oocyte
media_common.quotation_subject
Gene Expression
Receptor, Macrophage Colony-Stimulating Factor
Natriuretic peptide receptor 2
lcsh:Gynecology and obstetrics
Human chorionic gonadotropin
03 medical and health sciences
Mice
0302 clinical medicine
Ovarian Follicle
Internal medicine
medicine
Animals
Meiosis resumption
RNA, Messenger
Ovarian follicle
Equine chorionic gonadotropin
Ovulation
lcsh:RG1-991
media_common
030219 obstetrics & reproductive medicine
Germinal vesicle
urogenital system
Chemistry
Macrophage colony-stimulating factor
Research
Ovary
Obstetrics and Gynecology
NPR2
Meiosis
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Oncology
Oocytes
Female
Receptors, Atrial Natriuretic Factor
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 17572215
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Ovarian Research
- Accession number :
- edsair.doi.dedup.....b806b278c84b5c85c3588d6de9314aa4
- Full Text :
- https://doi.org/10.1186/s13048-017-0364-x