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Modulation of Phase II Enzymes by Sulforaphane: Implications for Its Cardioprotective Potential
- Source :
- Journal of Agricultural and Food Chemistry. 57:5615-5622
- Publication Year :
- 2009
- Publisher :
- American Chemical Society (ACS), 2009.
-
Abstract
- Oxidative stress plays a major role in the pathophysiology of cardiac disorders, but the experimental data on the protective effects of exogenous antioxidants are controversial. A promising cardioprotective strategy may be through the induction of the endogenous antioxidants and phase II enzymes by chemical inducers. Sulforaphane is an isothiocyanate derived from cruciferous vegetables, and it has gained attention mainly as a potential chemopreventive agent in part through the induction of detoxifying enzymes. Accordingly, this study was undertaken to investigate the time-dependent induction of gene transcription, protein expression, and enzyme activity of antioxidant and phase II enzymes [glutathione reductase, glutathione-S-transferase, glutathione peroxidase, NAD(P)H:quinone oxidoreductase-1, thioredoxin reductase] by sulforaphane in cultured rat neonatal cardiomyocytes. The potential cardioprotective action of sulforaphane was confirmed by the decrease in intracellular reactive oxygen species production, the increase in cell viability, and the decrease in DNA fragmentation after long-term treatment accompanied by the induction of antioxidants and phase II enzymes in cardiomyocytes.
- Subjects :
- Cardiotonic Agents
Antioxidant
Cell Survival
PHASE II ENZYMES
Thioredoxin reductase
medicine.medical_treatment
Glutathione reductase
medicine.disease_cause
CARDIOMYOCYTES
Gene Expression Regulation, Enzymologic
chemistry.chemical_compound
Enzyme activator
Isothiocyanates
medicine
Animals
Humans
Myocytes, Cardiac
Quinone Reductases
Cells, Cultured
Glutathione Transferase
chemistry.chemical_classification
Glutathione Peroxidase
Glutathione peroxidase
SULFORAPHANE
General Chemistry
Metabolic Detoxication, Phase II
Rats
Enzyme Activation
Oxidative Stress
Glutathione Reductase
Biochemistry
chemistry
Cardiovascular Diseases
Sulfoxides
Isothiocyanate
Reactive Oxygen Species
General Agricultural and Biological Sciences
Thiocyanates
Oxidative stress
Sulforaphane
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....b838bf9f8570009af90b53e2df3e5aa9