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Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
- Source :
- International Journal of Nanomedicine
- Publication Year :
- 2014
- Publisher :
- Dove Medical Press, 2014.
-
Abstract
- Yuri D Ivanov,1 Natalia S Bukharina,1 Tatyana O Pleshakova,1 Pavel A Frantsuzov,1 Elena Yu Andreeva,1 Anna L Kaysheva,1,2 Victor G Zgoda,1 Alexander A Izotov,1 Tatyana I Pavlova,1 Vadim S Ziborov,1 Sergey P Radko,1 Sergei A Moshkovskii,1 Alexander I Archakov1 1Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia; 2PostgenTech Ltd., Moscow, Russia Abstract: Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach was based on the AFM fishing of gp120 from the analyte solution using anti-gp120 aptamers immobilized on the AFM chip to count gp120/aptamer complexes that were formed on the chip surface. The comparison of image contrasts of fished gp120 against the background of immobilized aptamers and anti-gp120 antibodies on the AFM images was conducted. It was shown that an image contrast of the protein/aptamer complexes was two-fold higher than the contrast of the protein/antibody complexes. Mass spectrometry identification provided an additional confirmation of the target protein presence on the AFM chips after biospecific fishing to avoid any artifacts. Keywords: gp120 HIV-1 envelope glycoprotein, aptamer, atomic force microscopy, mass spectrometry
- Subjects :
- Analyte
Aptamer
viruses
gp120 HIV-1 envelope glycoprotein
Biophysics
Human immunodeficiency virus (HIV)
Immobilized Nucleic Acids
Pharmaceutical Science
Molecular Probe Techniques
Bioengineering
Nanotechnology
HIV Envelope Protein gp120
Mass spectrometry
medicine.disease_cause
Microscopy, Atomic Force
Mass Spectrometry
Biomaterials
International Journal of Nanomedicine
Drug Discovery
medicine
Original Research
atomic force microscopy
Chemistry
Atomic force microscopy
Organic Chemistry
technology, industry, and agriculture
aptamer
General Medicine
Aptamers, Nucleotide
Image contrast
Target protein
Subjects
Details
- Language :
- English
- ISSN :
- 11782013 and 11769114
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi.dedup.....b85b3905d7a08ce2e5c1bacac90938bf