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Neurobehavioral alterations plus transcriptional changes of the heat shock protein 90 and hypoxia inducible factor-1α in the crucian carp exposed to copper
- Source :
- NeuroToxicology. 52:162-175
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The various physiological alterations caused by copper (Cu) exposure in fish have attracted great interests toward neuronal strategies against Cu toxicity. Here, neurobehavioral activities (including anxiety-like behaviors) and transcriptional levels of heat shock protein (Hsp)90 and hypoxia inducible factor-1alpha (HIF-1α) were evaluated in the crucian carp (Carassius carassius) treated with nominal sub-lethal higher (1.45mg/L) and lower (0.30mg/L) concentrations of CuCl2. Both concentrations accounted for diminished swimming speed and food intake plus a strong preference for the dark side of the light/dark apparatus together with a reduction of crossings between the two compartments with respect to controls. Contextually, Hsp90 and HIF-1α transcripts were largely down- and up-regulated, respectively, in some brain areas such as in the medial part of the dorsal telencephalon (Dm, -52%) and in the ventral part of the ventral telencephalon (Vv, +68%). When carps were transferred to CuCl2-free water, some behaviors were rescued especially for fish previously exposed to 0.30mg/L concentration. In this same condition, Hsp90 mRNA levels were recuperated (-94%) in the medial preglomerular nucleus (NPGm) with respect to exposed fish while HIF-1α mRNA was mostly down-regulated in telencephalic stations. Moreover, recovery capacities of this extraordinary resistant fish was exhibited by evident reductions (-80%) of the dark argyrophilic granules such as in the ventral telencephalon (VTel). Overall, our results point to interesting responses against Cu toxicity involving Hsp90 and HIF-1α transcripts that may constitute early indicators of environmental stressors leading to the repair of metal-induced damages in fish with notable brain plasticity properties.
- Subjects :
- medicine.medical_specialty
Carps
Carassius carassius
Down-Regulation
AMPA receptor
010501 environmental sciences
Toxicology
01 natural sciences
03 medical and health sciences
0302 clinical medicine
Internal medicine
Heat shock protein
medicine
Animals
HSP90 Heat-Shock Proteins
0105 earth and related environmental sciences
Messenger RNA
Behavior, Animal
Dose-Response Relationship, Drug
biology
Cerebrum
General Neuroscience
Brain
Hypoxia (medical)
Hypoxia-Inducible Factor 1, alpha Subunit
biology.organism_classification
Hsp90
Up-Regulation
medicine.anatomical_structure
Endocrinology
Nerve Degeneration
Crucian carp
biology.protein
medicine.symptom
Copper
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 0161813X
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- NeuroToxicology
- Accession number :
- edsair.doi.dedup.....b894ee93553aa11c2cc0d4ee63bafe22
- Full Text :
- https://doi.org/10.1016/j.neuro.2015.12.006