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miR-375 exhibits a more effective tumor-suppressor function in laryngeal squamous carcinoma cells by regulating KLF4 expression compared with simple co-transfection of miR-375 and miR-206
- Source :
- Oncology Reports. 36:952-960
- Publication Year :
- 2016
- Publisher :
- Spandidos Publications, 2016.
-
Abstract
- MicroRNAs (miRNAs) are reported to be important regulators of cancer-related processes, and function either as oncogenes or as tumor-suppressor genes. It was found that miR-375 was downregulated in samples of laryngeal squamous cell carcinomas (LSCCs) as compared to the level noted in adjacent non-tumor tissues, and it was inversely correlated with T grade, lymph node metastases and clinical tumor stage. Overexpression of miR-375 led to a decreased protein level of Kruppel-like factor 4 (KLF4) and marked suppression of the proliferation and invasion, and induced apoptosis of LSCC cell line Hep-2 using Cell Counting Kit-8, Transwell chamber and cell cycle assays. In addition, we examined the influence of the upregulation of miR-206 alone and upregulation of both miR-375 and miR-206 on the expression of KLF4 and Hep-2 cell behavior. The results showed that compared with the function of miR-375 in tumor suppression by regulating KLF4, co-transfection of miR-375 and miR-206 exhibited a less effective inhibitory effect not only on tumor cell proliferation and invasion, but also on tumor cell apoptosis. Taken together, miR-375 is possibly a tumor suppressor in LSCC by regulating KLF4. In addition, simple overexpression of several miRNAs did not entail higher efficacy than a single miRNA, similar to co-transfecions of miR-375 and miR-206.
- Subjects :
- Male
0301 basic medicine
Cancer Research
Cell
Kruppel-Like Transcription Factors
Down-Regulation
Apoptosis
Biology
Transfection
Kruppel-Like Factor 4
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Mir-375
Cell Line, Tumor
microRNA
medicine
Humans
Genes, Tumor Suppressor
Neoplasm Invasiveness
Laryngeal Neoplasms
Cell Proliferation
Retrospective Studies
Oncogene
Cell Cycle
General Medicine
Middle Aged
Cell cycle
Up-Regulation
Squamous carcinoma
Cell biology
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Oncology
KLF4
Lymphatic Metastasis
030220 oncology & carcinogenesis
Carcinoma, Squamous Cell
Cancer research
Female
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....b8a29e2bdb2449d2b1355937730c8472